ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Mohammad Yunoos, Ch. Bharadwaj, V. Sandeep, S. Rajesh, Ch. Krishna

Email(s): yunoosvja@gmail.com

DOI: 10.5958/0974-4150.2015.00041.3   

Address: Mohammad Yunoos *, Ch. Bharadwaj, V. Sandeep, S. Rajesh, Ch. Krishna
Department of Pharmaceutical Analysis, Bapatla College of Pharmacy, Bapatla Andhra Pradesh.
*Corresponding Author

Published In:   Volume - 8,      Issue - 4,     Year - 2015


ABSTRACT:
Itraconazole is potent triazole anti-fungal agent that is prescribed to patients with fungal infections for the treatment of mycoses. In the present study a RP-HPLC method was developed and validated for the assay of itraconazole in bulk and its pharmaceutical dosage forms. The chromatographic system was equipped with Zorbax C18 column (150× 4.6 mm, 5µm). The HPLC system used was Agilent 1100 series. The detection was carried out using PDA detector at 262 nm with a mobile phase composition of acetonitrile and water in the ratio (75:25 %v/v) at a flow rate of 1.0 ml/min and the injection volume was 10 µl with 7 minutes runtime. The proposed method was linear over a concentration range of 4-40 µg/ml for the assay of itraconazole with a correlation coefficient of 0.9993. The retention time of itraconazole was found to be 3.753 min. The limit of detection and limit of quantification was found to be 0.624 µg/ml and 2.26 µg/ml respectively. The parameters of analysis were validated as per ICH guidelines. The results of the study showed that the proposed RP-HPLC method was simple, rapid, precise and accurate, which is useful for the routine estimation of itraconazole in bulk and pharmaceutical dosage forms.


Cite this article:
Mohammad Yunoos, Ch. Bharadwaj, V. Sandeep, S. Rajesh, Ch. Krishna. Determination of Itraconazole in Bulk and Capsule Dosage Form by a Validated Isocratic RP-HPLC Method. Asian J. Research Chem 8(4): April 2015; Page 236-240 doi: 10.5958/0974-4150.2015.00041.3


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