0974-4150 (Online)
0974-4169 (Print)

Author(s): Prasanna Reddy Battu, MS Reddy


DOI: Not Available

Address: Prasanna Reddy Battu1* and MS Reddy2
1Dept. Quality Control and Analytical R and D, Smilax Laboratories Limited, Jeedimetla, Hyderabad-500055, A.P, India.
2Department of Entomology and Plant Pathology, Auburn University, USA
*Corresponding Author

Published In:   Volume - 2,      Issue - 1,     Year - 2009

A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Rebeprazole sodium. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5µ , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (70:30, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 228 nm. The retention time of Rabeprazole sodium was 2.44 min. The method is accurate (99.15-101.85%), precise (intra-day variation 0.13-1.56% and inter-day variation 0.30-1.60%) and linear within range 0.1-30µ g/ml (R2=0.999) concentration and was successfully used in monitoring left over drug. The detection limit of Rabeprazole sodium at a signal-to- noise ratio of 3 was 1.80ng/ml in human plasma while quantification limit in human serum was 5.60 ng/ml. The proposed method is applicable to routine analysis of Rabeprazole sodium in pharmaceutical formulations as well as in human plasma samples.

Cite this article:
Prasanna Reddy Battu, MS Reddy. Development and Validation of RP-HPLC for the Rabeprazole sodium in Pharmaceutical formulations and Human Plasma. Asian J. Research Chem. 2(1): Jan.-March, 2009;Page 49-51.

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