Author(s):
Vikash Kumar Mehta, Rajkeshwar Prasad, Arvind Kumar Srivastava, Sudheer Kumar, Rohit Tripathi
Email(s):
rohit.tripathi783@gmail.com
DOI:
10.52711/0974-4150.2025.00034 
Address:
Vikash Kumar Mehta1, Rajkeshwar Prasad2, Arvind Kumar Srivastava2, Sudheer Kumar3, Rohit Tripathi2*
1Department of Pharmacognosy, Shambhunath Institute of Pharmacy, Jhalwa, Prayagraj, U.P., India - 211012.
2Department of Pharmaceutical Chemistry, Shambhunath Institute of Pharmacy, Jhalwa, Prayagraj,
U.P., India - 211012.
3Department of Biotechnology, National Institute of Pharmaceutical Education and Research Hajipur,
Bihar, India - 844102.
*Corresponding Author
Published In:
Volume - 18,
Issue - 4,
Year - 2025
ABSTRACT:
Objective: This study aims to evaluate the green synthesis of gold nanoparticles, characterisation and evaluation of anticancer activity by using the extract of Anethum graveolens aerial parts against hepatocellular carcinoma (HCC) cells, in-vitro model with docking approach. Methods: Gold nanoparticles were synthesized using an extract from the aerial portions of Anethum graveolens, followed by preliminary phytochemical screening and characterization by analytical methods as UV, FTIR, Zeta potential, XRD and SEM. The in-vitro anticancer activity against the HepG2 liver cancer cell line was assessed using the MTT assay, while the synthesized gold nanoparticles and plant extract was employed to investigate antioxidant activity by FRAP and DPPH methods. Results: Phytochemicals such as steroids, alkaloids, phenols, flavonoids, saponins etc was identified in Anethum graveolens aerial parts extract. UV range for plants extract flavonoids is typically 211-328 (nm) and their synthesized AuNPs showed broad peak at 538nm. FTIR for plant extract and their AG-AuNPs showed a broad peak around 3200-3500 cm-1 typically corresponds to O-H stretching vibrations and 1600-1750 cm-1 may reveal carbonyl (C=O) stretching. The XRD analysis of AG-AuNPs The XRD analysis of AG-AuNPs by Bragg"s reflection from (111), (200), (220), (222) and (311) FCC (face center cubic) crystal structure planes prove the crystalline nature of synthesized AuNPs. FE-SEM was prove the particle size and shape of AuNPs within the range of 10nm to 100nm with spherical in shape and presence of Au characteristic peaks at 2.12keV. The zeta potential value was found to be -20.38 indicate the neutral nature and stability of AuNPs. Total phenolic content (TPC) for plant extracts was found to be 64mg/g, and total flavonoid content (TFC) was 44.88mg/g. In antioxidant assay as DPPH and FRAP, the IC50 values of DPPH assay for a plant extract was found to be 58.33 and their AuNPs was 23.36. For FRAP assay the concentration of sample in (µg/ml) for plant extracts was found to be 46µg/ml and their AuNPs was found to be 78µg/ml. In-vitro anticancer assay of AG-AuNPs against human cancer cell lines showed variations in responsiveness to gold nanoparticles, with significant activity against liver cancer cell lines. Furthermore, the MTT assay revealed a concentration-dependent cytotoxic effect of the AuNPs on HepG2 cells, with an IC50 value of 113.9µg/ml indicating the effective inhibition of half of the HepG2 cell inhibition at this concentration in comparison to extract IC50 value of 155.5µg/ml.
Cite this article:
Vikash Kumar Mehta, Rajkeshwar Prasad, Arvind Kumar Srivastava, Sudheer Kumar, Rohit Tripathi. Novel-Biosynthesis and Characterization of Anethum graveolens gold Nanoparticles: In-vitro activity against Hepatocellular Carcinoma with Molecular Docking Approach. Asian Journal of Research in Chemistry.2025; 18(4):217-7. doi: 10.52711/0974-4150.2025.00034
Cite(Electronic):
Vikash Kumar Mehta, Rajkeshwar Prasad, Arvind Kumar Srivastava, Sudheer Kumar, Rohit Tripathi. Novel-Biosynthesis and Characterization of Anethum graveolens gold Nanoparticles: In-vitro activity against Hepatocellular Carcinoma with Molecular Docking Approach. Asian Journal of Research in Chemistry.2025; 18(4):217-7. doi: 10.52711/0974-4150.2025.00034 Available on: https://ajrconline.org/AbstractView.aspx?PID=2025-18-4-3
REFERENCES:
1. Bosch FX, Ribes J, Díaz M, Cléries R. Primary liver cancer: worldwide incidence and trends. Gastroenterology. 2004 Nov 1;127(5): S5-16.
2. Chahal KK, Monika AK, Bhardwaj U, Kaur R. Chemistry and biological activities of Anethum graveolens L.(dill) essential oil: A review. Journal of pharmacognosy and phytochemistry. 2017;6(2):295-306.
3. Clogston JD, Patri AK. Zeta potential measurement. InCharacterization of nanoparticles intended for drug delivery 2010 Oct 19 (pp. 63-70). Totowa, NJ: Humana press.
4. Czeczot H, Tudek B, Kusztelak J, Szymczyk T, Dobrowolska B, Glinkowska G, Malinowski J, Strzelecka H. Isolation and studies of the mutagenic activity in the Ames test of flavonoids naturally occurring in medical herbs. Mutation Research/Genetic Toxicology. 1990 Mar 1;240(3):209-16.
5. Ghany MG, Kleiner DE, Alter H, Doo E, Khokar F, Promrat K, Herion D, Park Y, Liang TJ, Hoofnagle JH. Progression of fibrosis in chronic hepatitis C. Gastroenterology. 2003 Jan 1;124(1):97-104.
6. Grela E, Kozłowska J, Grabowiecka A. Current methodology of MTT assay in bacteria–A review. Acta histochemica. 2018 May 1;120(4):303-11.
7. Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutrition research. 2003 Dec 1;23(12):1719-26.
8. Haiss W, Thanh NT, Aveyard J, Fernig DG. Determination of size and concentration of gold nanoparticles from UV−Vis spectra. Analytical chemistry. 2007 Jun 1;79(11):4215-21.
9. Huang X, Devi S, Bordiga M, Brennan CS, Xu B. Phenolic compounds mediated biosynthesis of gold nanoparticles and evaluation of their bioactivities: a review. International Journal of Food Science and Technology. 2023 Apr;58(4):1673-94.
10. Jana S, Shekhawat GS. Anethum graveolens: An Indian traditional medicinal herb and spice. Pharmacognosy reviews. 2010 Jul;4(8):179.
11. Kubiak K, Adamczyk Z, Maciejewska J, Oćwieja M. Gold nanoparticle monolayers of controlled coverage and structure. The Journal of Physical Chemistry C. 2016 Jun 9;120(22):11807-19.
12. Liu H, Zhang X, Xu Z, Wang Y, Ke Y, Jiang Z, Yuan Z, Li H. Role of polyphenols in plant-mediated synthesis of gold nanoparticles: identification of active components and their functional mechanism. Nanotechnology. 2020 Jul 17;31(41):415601.
13. Lončarić A, Celeiro M, Jozinović A, Jelinić J, Kovač T, Jokić S, Babić J, Moslavac T, Zavadlav S, Lores M. Green extraction methods for extraction of polyphenolic compounds from blueberry pomace. Foods. 2020 Oct 23;9(11):1521.
14. Losic B, Craig AJ, Villacorta-Martin C, Martins-Filho SN, Akers N, Chen X, Ahsen ME, Von Felden J, Labgaa I, DʹAvola D, Allette K. Intratumoral heterogeneity and clonal evolution in liver cancer. Nature communications. 2020 Jan 15;11(1):291.
15. Mahakham W, Theerakulpisut P, Maensiri S, Phumying S, Sarmah AK. Environmentally benign synthesis of phytochemicals-capped gold nanoparticles as nanopriming agent for promoting maize seed germination. Science of the Total Environment. 2016 Dec 15; 573:1089-102.
16. Othman A, Mukhtar NJ, Ismail NS, Chang SK. Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. International Food Research Journal. 2014 Mar 1;21(2):759.
17. Pallab K, Tapan B, Tapas P, Ramen K. Estimation of total flavonoids content (TPC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug delivery and Therapeutics. 2013;3(4):33-7.
18. Paul P, Chatterjee S, Pramanik A, Karmakar P, Chandra Bhattacharyya S, Kumar GS. Thionine conjugated gold nanoparticles trigger apoptotic activity toward HepG2 cancer cell line. ACS Biomaterials Science & Engineering. 2018 Feb 12;4(2):635-46.
19. Peng C, Ye Z, Na J, Liu X, Zhang Z. Establishment and refinement of a DEN-induced hepatocellular carcinoma model in rats. Oncologie. 2024 May 27;26(3):419-31.
20. Petibois C, Cazorla G, Gin H, Déléris G. Differentiation of populations with different physiologic profiles by plasma Fourier-transform infrared spectra classification. Journal of Laboratory and Clinical Medicine. 2001 Mar 1;137(3):184-90.
21. Qawy A, Fawzy MA, Ali A, Abdel-Latif MM, Eltahir HM, Elbadawy HM, Alahmadi YM, Mohafz O, Abouzied MM, Nazmy MH. P53 and endoglin, two biomarkers for predicting HBV and hepatocellular carcinoma in Egyptian patients. Journal of Advanced Biomedical and Pharmaceutical Sciences. 2020 Apr 1;3(2):110-5.
22. Rumpf J, Burger R, Schulze M. Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules. 2023 Apr 1; 233:123470.
23. Sakurai T, Kudo M, Umemura A, He G, Elsharkawy AM, Seki E, Karin M. p38α inhibits liver fibrogenesis and consequent hepatocarcinogenesis by curtailing accumulation of reactive oxygen species. Cancer research. 2013 Jan 1;73(1):215-24.
24. Samatha T, Shyamsundarachary R, Srinivas P, Swamy NR. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian Journal of Pharmaceutical and Clinical Research. 2012 Nov 1;5(4):177-9.
25. Saravanan GO, Alagarsamy VE, Prakash CR. Synthesis and evaluation of antioxidant activities of novel quinazoline derivatives. Int J Pharm Pharm Sci. 2010;2(4):83-6.
26. Turati F, Galeone C, Rota M, Pelucchi C, Negri E, Bagnardi V, Corrao G, Boffetta P, La Vecchia C. Alcohol and liver cancer: a systematic review and meta-analysis of prospective studies. Annals of oncology. 2014 Aug 1;25(8):1526-35.
27. Verma A. Lead finding from Phyllanthus debelis with hepatoprotective potentials. Asian Pacific Journal of Tropical Biomedicine. 2012 Jan 1;2(3): S1735-7.
28. Verma HN, Singh P, Chavan RM. Gold nanoparticle: synthesis and characterization. Veterinary world. 2014 Feb 1;7(2):72.
29. Yan W, Petkov V, Mahurin SM, Overbury SH, Dai S. Powder XRD analysis and catalysis characterization of ultra-small gold nanoparticles deposited on titania-modified SBA-15. Catalysis Communications. 2005 Jun 1;6(6):404-8
30. Ye B, Tian W, Wang B, Liang J. CASTpFold: Computed Atlas of Surface Topography of the universe of protein Folds. Nucleic acids research. 2024 Jul 5;52(W1): W194-9.