ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Bhope SG, Kuber VV, Patil MJ, Ghosh VK

Email(s): bshrinivas16@gmail.com

DOI: Not Available

Address: Bhope SG1*, Kuber VV2, Patil MJ3 and Ghosh VK4
1Group Leader- Analytical R and D, Tulip Lab Pvt. Ltd. F-20/21, MIDC Ranjangaon, Pune 412220, INDIA
2Head, R and D, Tulip Lab Pvt. Ltd. F-20/21, MIDC Ranjangaon, Pune412220, INDIA
3Marathwada Mitra Mandal College of Pharmacy, Pune, INDIA
4Research Scientist- Analytical R and D, Tulip Lab Pvt. Ltd. F-20/21, MIDC Ranjangaon, Pune412220, INDIA
*Corresponding Author

Published In:   Volume - 2,      Issue - 3,     Year - 2009


ABSTRACT:
This paper describes a new, simple, precise, and accurate HPTLC method for quantitation of andrographolide in kalmegh extract and its pharmaceutical dosage form. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of toluene: ethyl acetate: formic acid : methanol 50:30:05:2.5 (v/v/v/v). Densitometric evaluation of the separated zones was performed at 226 nm. The andrographolide was satisfactorily resolved with RF values of 0.34 ± 0.03 in both plant extract and pharmaceutical dosage form. The accuracy and reliability of the method was assessed by evaluation of linearity (100–800 ng spot-1), precision (method precision RSD 1.52% and system precision RSD 1.38%), accuracy (97.34 ± 1.47) and specificity in accordance with ICH guidelines.


Cite this article:
Bhope SG, Kuber VV, Patil MJ, Ghosh VK. Validated HPTLC Method for the Quantitation of Andrographolide from Raw Material and Pharmaceutical Dosage Form. Asian J. Research Chem. 2(3): July-Sept., 2009, page 314-317.


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