ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Kempohalli S. Chandrashekharaiah

Email(s): kschandraks@gmail.com

DOI: Not Available

Address: Kempohalli S. Chandrashekharaiah*
Department of Biotechnology, PES Institute of Technology, BSK III stage, Bangalore-560085, Karnataka, India.
*Corresponding Author

Published In:   Volume - 5,      Issue - 11,     Year - 2012


ABSTRACT:
The proteins and chymotrypsin inhibitors were isolated and studied from the seeds of eight varieties of Mucuna. The total protein content varied from 15 – 26%. The seeds of all the varieties of Mucuna exhibited chymotrypsin inhibitor activity, varied from 3 – 7 CIA/mg of protein. There was no much variation in the chynotrypsin inhibitory activity among soaked seeds and dry seeds. The Mucuna seeds were germinated for six days and change in the total protein content and chymotrypsin inhibitors were monitored. The total protein content and chymotrypsin inhibitory activity of the endosperm showed reciprocal variation, such that, the protein content increased up to 72 hrs of germination and then decreased followed by decreased chymotrypsin inhibitory activity with increase in germination time. The chymotrypsin inhibitor activity was decreased from 6.78 CIA/mg to 1.24 CIA/mg (81% CIA reduction after 144 hrs germination). The proteins were numbered from anodic end. The protein banding pattern revealed the presence of total 11 bands on anionic polyacrylamide gel electrophoresis. Mucuna cochinensis and Mucuna cochinensis MP9 were identical in both number of bands (total of 09 bands) and banding pattern. Electrophoretic analysis of chymotrypsin inhibitors revealed the presence of five to seven isoinhibitors in the seeds of different varieties of Mucuna. The Mucuna hirsute and Mucuna pruriens contained a maximum of seven chymotrypsin isoinhibitors.


Cite this article:
Kempohalli S. Chandrashekharaiah. Studies on variation and stability of storage proteins and chymotrypsin inhibitors of an underutilized legume, Mucuna during germination. Asian J. Research Chem. 5(11): Nov., 2012; Page 1388-1391.

Cite(Electronic):
Kempohalli S. Chandrashekharaiah. Studies on variation and stability of storage proteins and chymotrypsin inhibitors of an underutilized legume, Mucuna during germination. Asian J. Research Chem. 5(11): Nov., 2012; Page 1388-1391.   Available on: https://ajrconline.org/AbstractView.aspx?PID=2012-5-11-14


Recomonded Articles:

Author(s): Anitha Jebamalai Raj, Sudarsanam Dorairaj

DOI:         Access: Open Access Read More

Author(s): Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V

DOI: 10.5958/0974-4150.2019.00062.2         Access: Open Access Read More

Author(s): Shlini P., Siddalinga Murthy K.R.

DOI:         Access: Open Access Read More

Author(s): A. Anita, A. Malar Retna, J. Joseph

DOI: 10.5958/0974-4150.2018.00019.6         Access: Open Access Read More

Author(s): Reena Bhadani, S. K. Sen Gupta, U. K. Mitra

DOI: 10.5958/0974-4150.2018.00021.4         Access: Open Access Read More

Author(s): K. Natarajan, S. Tamilarasi, R. Sathish, C. Amudhadevi, T. Regupathi;

DOI:         Access: Open Access Read More

Author(s): T. Shanthi, S. Sathiyaraj, S. Rajendran, J. Sathiyabama

DOI: 10.5958/0974-4150.2018.00148.7         Access: Open Access Read More

Author(s): T. A. Rajput, A.P. Rajput, M. V. Girase, S. N. Patel

DOI:         Access: Open Access Read More

Author(s): M. Padmaja, B. Praveen, B. Raghunath Reddy, P. Venu Madhav

DOI:         Access: Open Access Read More

Author(s): Avanti K. Galande, Sachin H. Rohane

DOI: 10.5958/0974-4150.2021.00017.1         Access: Closed Access Read More

Author(s): Savitha A.S.*, Naik M.K. , Ajithkumar K.

DOI:         Access: Open Access Read More

Author(s): Yatri R Shah, Dhrubo Jyoti Sen, CN Patel

DOI:         Access: Open Access Read More

Author(s): Njoku Obioma U., Joshua, Parker Elijah, Ossai Emmanuel C., Agu, Chidozie Victor, Ugwuanyi Juliana O.

DOI:         Access: Open Access Read More

Author(s): Njoku Obioma U., Joshua Parker Elijah, Okonkwo Chukwudi C;

DOI:         Access: Open Access Read More

Author(s): Swati Korabu, Rajani Nair, Chaitrali Pawar, Rohit Shah, Priyanka Jadhav, Bhakti Chorghe

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags