Author(s):
Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V
Email(s):
gskpungai@gmail.com
DOI:
10.5958/0974-4150.2019.00062.2 
Address:
Gopalasatheeskumar K1, Ariharasiva Kumar G2, Sengottuvel T3, Sanish Devan V4, Srividhya V5
1Research Scholar, Department of Pharmacy, Annamalai University, Chidambaram, Tamil Nadu, India.
2Professor and Head of the Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
3Associate Professor, Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
4Department of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
5Assistant Professor, Department of Pharmacology, Excel College of Pharmacy, Pallakapalayam, Namakkal-
637303 (DT), Tamil Nadu, India (Affiliated to The Tamil Nadu Dr. M.G.R. Medical University).
Published In:
Volume - 12,
Issue - 6,
Year - 2019
ABSTRACT:
Plants and plant-derived products contain a wide range of phytochemicals, such as alkaloids, flavanoids, glycosides which are thought to have the medicinal properties. Cucumis melo var agrestis is belongs to the family of cucurbitaceae and well distributed in rural and coastal areas. This plant is consumed by rural people in Tamil Nadu, India. Phenolic and flavanoid contents plays the important role in antioxidant mechanism thereby cure many of the diseases. Therefore current research is aimed to quantification of total phenolic and flavanoid content present in leaves of CMA. Hydroalcoholic leaf extract of CMA (HALEC) was evaluated its phytochemicals using chemical tests and quantification of total phenolic content and total flavonoid contents by standard curve of Gallic acid and Quercetin using UV- Spectrophotometer. Our results revealed that HALEC has the phytochemicals like alkaloids, flavonoids, tannins, carbohydrates, saponins, glycosides, proteins and amino acids and quantitative tests confirms the presence of total phenolic content was 77.82 mg/g of extract calculated as Gallic acid equivalent and total flavonoid content was 30.06 mg/g of extract calculated as Gallic acid equivalent in HALEC. This research work is useful to animal study on leaf extract of CMA, Isolation of active principles and detection of exact mechanism.
Cite this article:
Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V. Quantification of Total Phenolic and Flavonoid content in leaves of Cucumis melo var agrestis using UV- spectrophotometer. Asian J. Research Chem. 2019; 12(6):335-337. doi: 10.5958/0974-4150.2019.00062.2
Cite(Electronic):
Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V. Quantification of Total Phenolic and Flavonoid content in leaves of Cucumis melo var agrestis using UV- spectrophotometer. Asian J. Research Chem. 2019; 12(6):335-337. doi: 10.5958/0974-4150.2019.00062.2 Available on: https://ajrconline.org/AbstractView.aspx?PID=2019-12-6-10