ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): M. Ramesh, M. Kanaka Durga, Sravani A., T. Snehalatha, Thimmareddy D.

Email(s): kanakadurga.pharma@gmail.com

DOI: Not Available

Address: M. Ramesh, M. Kanaka Durga*, Sravani A., T. Snehalatha, Thimmareddy D.
Department of Pharmaceutical Analysis and Quality Assurance, Vijaya college of Pharmacy, Munaganoor, Hayathnagar, Hyderabad
*Corresponding Author

Published In:   Volume - 5,      Issue - 8,     Year - 2012


ABSTRACT:
A simple, rapid, specific, accurate and precise stability indicating reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Azithromycin and Cefixime in Bulk and Tabletdosage form. An Agilant Zorbax C8, 5 µ column having 150 x 4.6mm id in Isocratic mode with mobile phase containing 0.1M Dipotassium Hydrogen Phosphate Buffer : methanol (60:40 %v/v pH: 8.0 adjusted with Ortho phosphoric acid) was used. The flow rate was 1ml/min and effluents were monitored at 230nm. The retention time of Azithromycin and Cefixime was 2.7min and 4.6min respectively. The concentration curves were linear in the concentration range of 250-750µg/mL and 200-600 µg/mL. The drug was subjected to acid and alkali hydrolysis, oxidation, photolysis and heat as stress conditions. The developed method was validated for specificity, precision, linearity, accuracy, LOD,LOQ, robustness. Recovery of Azithromycin and Cefixime in formulations was found to be in the range of 98% -102% and 98%-102% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and good precision, the proposed HPLC method may be used for the simultaneous determination of these two drugs in pharmaceutical dosage forms.


Cite this article:
M. Ramesh, M. Kanaka Durga, Sravani A., T. Snehalatha, Thimmareddy D.. A New Stability Indicating Validated RP-HPLC Method for the Simultaneous Estimation of Azithromycin and Cefixime in Bulk and Pharmaceutical Dosage Forms. Asian J. Research Chem. 5(8): August, 2012; Page 1067-1073.


Recomonded Articles:

Author(s): Bulusu Lakshmi Sushma1, Gutta Madhusudhan, Anireddy Jayashree

DOI: 10.5958/0974-4150.2017.00014.1         Access: Open Access Read More

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Alka Verma, Bhupesh Verma, Sunil Kumar Prajapati, Kishu Tripathi

DOI:         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): Banerjee S, Bonde CG, Merukar SS, Patil YR

DOI:         Access: Open Access Read More

Author(s): B. Balaswami, P. Venkata Ramana, B. Subba Rao, P. Sanjeeva

DOI: 10.5958/0974-4150.2018.00023.8         Access: Open Access Read More

Author(s): Ismail, R Rajavel, M Ganesh, M Jagadeeswaran, K Srinivasan, J Valarmathi, T Sivakumar

DOI:         Access: Open Access Read More

Author(s): Manorama B. Motegaonkar, S. D. Salunke

DOI: 10.5958/0974-4150.2017.00025.6         Access: Open Access Read More

Author(s): JR Rao, Mohan Kumar, SS Yadav

DOI:         Access: Open Access Read More

Author(s): Effat Souri, Nazanin Shabani Ravari, Farhad Alvandifar, Arsalan Negahban Aghdami, Maliheh Barazandeh Tehrani, Massoud Amanlou

DOI:         Access: Open Access Read More

Author(s): PA Jadhav, CS Raut, JP Bidada, BB Buwa, PN Dhabale, SC Dhawale

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags