ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): S. Hasan Amrohi, Mahesh Nasare, Afra Nazneen, Satish J.

Email(s): hasanpharma26@gmail.com

DOI: Not Available

Address: S. Hasan Amrohi*, Mahesh Nasare, Afra Nazneen, Satish J.
Department of Analytical Chemistry, School of Pharmacy, Anurag Group of Institutions, Hyderabad, A.P, India-501301.
*Corresponding Author

Published In:   Volume - 7,      Issue - 1,     Year - 2014


ABSTRACT:
A simple, specific, accurate and precise stability indicating reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of Aspirin and Isosorbide 5-mononitrate in bulk drug and its pharmaceutical dosage form. A chromatographic separation was achieved with reverse phase phenomenex Luna 5µ C18 (2) 100A (250 × 4.60 mm) column in an isocratic mode at ambient temperature. The mobile phase consisting of water: methanol: acetonitrile (55:28:17% v/v/v) at a flow rate of 1 ml/min. The eluents were monitored at 217 nm. The retention times of Aspirin and Isosorbide 5-mononitrate were found to be 2.05±0.056 min and 4.27±0.016 min respectively. The regression analysis revealed linearity in the concentration range of 1-10 µg/ml and 1-10 µg/ml for Aspirin and Isosorbide 5-mononitrate respectively. The method was validated in terms of linearity, accuracy, precision, and limit of detection (LOD), limit of quantification (LOQ) in accordance with ICH guide lines. The results of the study showed that the developed method is simple, rapid, precise and accurate, and therefore suitable for routine analysis of these drugs in pharmaceutical dosage form.


Cite this article:
S. Hasan Amrohi, Mahesh Nasare, Afra Nazneen, Satish J.. Development and Validation of Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Anti-Anginal Drugs in Pharmaceutical Dosage Form. Asian J. Research Chem 7(1): January 2014; Page 41-47.

Cite(Electronic):
S. Hasan Amrohi, Mahesh Nasare, Afra Nazneen, Satish J.. Development and Validation of Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Anti-Anginal Drugs in Pharmaceutical Dosage Form. Asian J. Research Chem 7(1): January 2014; Page 41-47.   Available on: https://ajrconline.org/AbstractView.aspx?PID=2014-7-1-7


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