The nucleation process is one of the most fascinating problems in protein crystallization. The mechanisms leading to the formation of clusters of molecules displaying translational and rotational order have their own rules completely different from those of crystal growth. This paper has proof about growth of protein crystallization by hanging drop method in which Langmuir–Blodgett (LB) protein thin film has been used as a substrate. In this method, the liquid droplet on Nano biofilm surfaces enhances the size and quality of crystal. Comparing with the traditional classic drop method, it can also reduce the evaporation rate of the liquid droplet and provide a stable environment for the crystal growth. In this work, crystal growth experiments for lysozyme and thaumatin proteins have shown the positive effect on rate of crystal growth. The features of this LB method and its mechanism have also been discussed.
Cite this article:
Manju Singh. Protein Crystallization: Nucleation and Growth on the Surface of Langmuir–Blodgett Nano Biofilm. Asian J. Research Chem. 7(2): February 2014; Page 214-219.