ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): G. Raveendra Babu, A. Lakshmana Rao, J. Venkateswara Rao

Email(s): dralrao@gmail.com

DOI: Not Available

Address: G. Raveendra Babu1, A. Lakshmana Rao2* and J. Venkateswara Rao3
1D.C.R.M. Pharmacy College, Inkollu- 523 167, A.P., India.
2V.V. Institute of Pharmaceutical Sciences, Gudlavalleru- 521 356, A.P., India.
3Sultan-Ul-Uloom College of Pharmacy, Hyderabad- 500 034, A.P., India.
*Corresponding Author

Published In:   Volume - 7,      Issue - 9,     Year - 2014


ABSTRACT:
A reverse phase liquid chromatographic method (RP-HPLC) was developed to estimate the amount of Desonide in bulk and its pharmaceutical formulations. Waters Alliance HPLC system equipped with auto sampler, UV-Visible detector and Altima C18 (100 x 4.6 mm, 5m) column were used for the quantification of the drug. Separation was carried out by using potassium dihydrogen phosphate buffer of pH 4.8 and acetonitrile in the ratio 45:55 v/v as mobile phase at a flow rate of 1 ml/min and the detection was carried out at a wavelength of 240 nm. The retention time, tailing factor and USP theoretical plates of Desonide were found to be 3.555 min., 1.19 and 3367 respectively. The area of the peak was proportional to the concentration of the drug in the range 2.5-15 µg/ml of Desonide. The values of LOD and LOQ for Desonide were found to be 0.040 and 0.121 µg/ml respectively. The mean recovery of the substance was found to be 100%. The developed method was found to be simple, repeatable and reproducible and hence it can be used as an alternative method in any pharmaceutical industries in the assay of Desonide.


Cite this article:
G. Raveendra Babu, A. Lakshmana Rao, J. Venkateswara Rao. Development and Validation of Stability-Indicating Reverse Phase Liquid Chromatographic Method for the Assay of Desonide in Bulk and Pharmaceutical Formulations. Asian J. Research Chem. 7(9): September 2014; Page 805-809.


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