Author(s):
Mimouna Hani, Chérifa Boubekri, Touhami Lanez
Email(s):
cherifa.boubekri@univ-biskra.dz
DOI:
10.52711/0974-4150.2023.00004 
Address:
Mimouna Hani1,3, Chérifa Boubekri2,3*, Touhami Lanez1,3
1University of Echahid Hamma Lakhdar, Faculty of Exact sciences, PO Box 789, 39000, El Oued, Algeria.
2University Mohamed Khider, Faculty of Exact sciences, Natural and Life Sciences,
Department of Material sciences, BP145 RP Biskra 07000 Algeria.
3VTRS Laboratory, B.P.789, 39000, El Oued, Algeria.
*Corresponding Author
Published In:
Volume - 16,
Issue - 1,
Year - 2023
ABSTRACT:
In this study, two solvents with different polarity were used in the extraction of the dried powdered roots of the plant Juncus rigidus; ethanol, acetone, during two different growth stages : ripening period (November) and flowering period (April). Total phenolics, total flavonoids, and total flavanols contents were evaluated using the colorimetric assays. The obtained results were very significant and showed that polarity of solvents and growth stage affects the composition of the plant. The highest content in total phenolic (87,249 ± 0.005 mg GAE/g), total flavonoid (28,109 ± 0.041 mg QE/g) and total flavonol (33,195 ± 0,013 mg GAE/g) contents was obtained in acetone extract during flowering period witch decrease during ripening period. The best result for the evaluation of the antioxidant activity indicated that highest value in DPPH free radical scavenging activity (0,014 ± 0,0003 mg/mL-1), phosphomolybdenum assay (425,168 ± 0,006 mL/mg), ferric reducing antioxidant power (1217,914 ± 0,022 mgFeSO4/g) and cyclic voltammetry (47,07 ± 0,04 mg/g) was unregistered during ripening stage using acetone solvent.
Cite this article:
Mimouna Hani, Chérifa Boubekri, Touhami Lanez. Mimouna Hani, Chérifa Boubekri, Touhami Lanez. Asian Journal of Research in Chemistry. 2023; 16(1):18-6. doi: 10.52711/0974-4150.2023.00004
Cite(Electronic):
Mimouna Hani, Chérifa Boubekri, Touhami Lanez. Mimouna Hani, Chérifa Boubekri, Touhami Lanez. Asian Journal of Research in Chemistry. 2023; 16(1):18-6. doi: 10.52711/0974-4150.2023.00004 Available on: https://ajrconline.org/AbstractView.aspx?PID=2023-16-1-4
REFERENCES:
1. El-Shamy AI. Abdel-Razek AF. Nassar MI. Phytochemical review of Juncus L. genus (Fam. Juncaceae). Arabian Journal of Chemistry 2015; 8:614-623.
2. Kovács A. Vasas A. Hohmann J. Natural phenanthrenes and their biological activity. Phytochemistry 2008; 69(5):1084-110.
3. Ghareeb MA. Refahy LA. Saad AM. Ahmed WS. Chemical composition, antioxidant and anticancer activities of the essential oil from Eucalyptus citriodora (Hook.) leaves. Der Pharma Chemica. 2016; 8(1):192-200.
4. Benarima A. Laouini SE. Ben Seghir B. Belaiche Y. Ouahrani MR. Optimization of Ultrasonic-Assisted Extraction of Phenolic Compounds from Moringa Oleifera Leaves using Response Surface Methodology. Asian Journal of Research in Chemistry. 2020; 13(5):307-311.
5. Shlini P. Siddalinga Murthy KR. Extraction of Phenolics, Proteins and Antioxidant Activity from Defatted Tamarind Kernel Powder. Asian Journal of Research in Chemistry. 2011; 4(6): 936-941.
6. Gopalasatheeskumar K. Ariharasiva Kumar G. Sengottuvel T. Sanish Devan V. Srividhya V. Quantification of Total Phenolic and Flavonoid content in leaves of Cucumis melo var agrestis using UV- spectrophotometer. Asian Journal of Research in Chemistry. 2019; 12(6):335-337.
7. Rajurkar RM. Jain RG. Bedmohta PA. Khadbadi SS. Antioxidant Activity of Phenolic Extract from Ginger (Zingiber officinale Roscoe) Rhizome. Asian Journal of Research in Chemistry. 2009; 2(3): 260-261.
8. Tiwari P. Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Amritarishta Prepared by Traditional and Modern Methods. Asian Journal of Research in Chemistry. 2013; 6(12): 1173-1178.
9. Messaoudi A. Dekmouche M. Rahmani Z. Bensaci C. Comparative Analysis of Total Phenolics, Flavonoid content and Antioxidant profile of date palm (Phoenix dactylifera L.) with different watering water from Oued Soufin Algeria; Asian Journal of Research in Chemistry. 2020; 13(1): 28-32.
10. Habchi A. Dekmouche A. Hamia C. Saidi M. Yousfi M. Bouguerra A. Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro. Asian Journal of Research in Chemistry. 2021; 14(3):161-7.
11. Zehiroglu C. Beyza S. and Sarikaya O. The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology. 2019; 56(11):4757–4774.
12. Isildak Ö. Yildiz I. Genc N. A new potentiometric PVC membrane sensor for the determination of DPPH radical scavenging activity of plant extracts. Food chemistry. 2021; 373: 131420.
13. Thaipong K. Boonprakob U. Crosby K. Cisneros-Zevallos L. Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 2006; 19: 669–675.
14. Li R. Guo M. Zhang G. Xu X. Li K. Neuroprotection of nicotiflorin in permanent focal cerebralischemia and in neuronal cultures. Biological and Pharmaceutical Bulletin. 2007; 29: 1868-1872.
15. Bahorun T. Gressier B. Trotin F. Brunet C. Dine T. Luyckx M. Vasseur J. Cazin M. Cazin JC. Pinkas M. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung. 1996; 46(11):1086-9.
16. Formagio ASN. Volobuff CRF. Santiago M. Cardoso CAL. Vieira MDS and Pereira ZE. Evaluation of antioxidant activity, total flavonoids, tannins and phenolic compounds in Psychotria leaf extracts. Antioxidants. 2014; 3: 745-757.
17. Burits M. Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research. 2000; 14: 323-328.
18. Cuendet M. Hostettmann K. Potterat O. Iridoidglucosides with free radical scavenging properties from Fagraea blumei. Helvitica Chimica Acta.1997; 80(4): 1144–1152.
19. Que F. Mao L. Pan X. Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Research International. 2006; 39 (5):581–587.
20. Amo-Mensah J. Darko G. Borquaye LS. Anti-inflammatory and antioxidant activities of the root and bark extracts of Vitex grandifolia (Verbanaceae). Scientific African. 2020; 10: e00586.
21. Labed B. Bouhrira A. Benaissa K. Gherraf N. Radical scavenging and Antioxidant activities of three extracts of Lavandula coronopifolia rown in El-Hoggar, Tamanrasset, Algeria. Der Pharma Chemica. 2016; 8(9):132-139.
22. Benzie IF. Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of ‘‘Antioxidant Power’’: The FRAP Assay. Analytical Biochemistry. 1996; 239: 70–76.
23. Fernandes RPP. Trindade MA. Tonin GF. Lima CG. Pugine SMP. Munekata PES. Lorenzo JM. De Melo MP. Evaluation of antioxidant capacity of 13 plant extracts by three different methods: cluster analyses applied for selection of the natural extracts with higher antioxidant capacity to replace synthetic antioxidant in lamb burgers. Journal of Food Science and Technology. 2016; 53(1): 451- 460.
24. Amidi S. Majob F. Moghaddem AB. Tabib K. Kobarfard F. A simple electrochemical method for the rapid estimation of antioxidant potentials of some selected medicinal plants. Iranian journal of pharmaceutical research. 2012; 11 (1): 117-121.
25. Guediri I. Boubekri C. Smara O. Lanez T. Total phenolic contents and determination of Antioxidant activity by DPPH, FRAP, and cyclic voltammetry of the fruit of Solanum nigrum (black nightshade) growing in the south of Algeria. Asian Journal of Research in Chemistry. 2021; 14(1):47-55.
26. López A. Rico M. Rivero A. Tangil MS. The effects of solvents on the phenolic contents and antioxidant activity of Stypocaulon scoparium algae extracts. Food Chemistry. 2011; 125: 1104–1109.
27. Zhao H. Dong J. Lu J. Chen J. Li Y. Shan L. Lin Y. Fan W. Gu G. Effect of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in Barley (Hordeum vulgare L.). Journal of Agricultural and Food Chemistry. 2006; 54: 7277–7286.
28. Musa KH. Abdullah A. Jusoh K. Subramaniam V. Antioxidant Activity of Pink-Flesh Guava (Psidium guajava L.): Effect of Extraction Techniques and Solvents. Food Analytical Methods. 2011; 4: 100-107.
29. Jacotet-Navarro M. Laguerre M. Fabiano-Tixier AS. Tenon M. Feuillère N. Bily A. Chemat F. What is the best ethanol-water ratio for the extraction of antioxidants from rosemary? Impact of the solvent on yield, composition, and activity of the extracts. Electrophoresis. 2018; 39:1946-1956.
30. Truong D. Nguyen DH. Ta NTA. Bui AV. Do TH. Nguyen HC. Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia. Journal of Food Quality. 2019; 1:1-9.
31. Mollaei S. Hazrati S. Lotfizadeh VD. Dastan D. Asgharian P. Phytochemical variation and biological activities of Zosima absinthifolia during various stages of growth. International Journal of Food Properties, 2020, 23(1), 1556-1567.
32. AlFaris NA. AlTamimi JZ. AlGhamdi FA. Albaridi NA. Alzaheb RA. Aljabryn DH. Aljahani AH. AlMousa LA. Total phenolic content in ripe date fruits (Phoenix dactylifera L.): A systematic review and meta-analysis. Saudi Journal of Biological Sciences.2021; 28:3566–3577.
33. Herrera-Pool E. Ramos-Díaz AL. Lizardi-Jiménez MA. Pech-Cohuo S. Ayora-Talavera T. Cuevas-Bernardino JC. García-Cruz U. Pacheco N. Effect of solvent polarity on the ultrasound assisted extraction and antioxidant activity of phenolic compounds from habanero pepper leaves (Capsicum chinense) and its identification by UPLC-PDA-ESI-MS/MS. Ultrasonics Sonochemistry. 2021; 76: 105658.
34. Ma YL. Sun P. Feng J. Yuan J. Wang Y. Shang YF. Niu XL. Yang SH. Wei ZJ. Solvent effect on phenolics and antioxidant activity of Huangshan Gongju (Dendranthema morifolium (Ramat) Tzvel. cv. Gongju) extract. Food and Chemical Toxicology. 2021; 147: 111875.
35. Mokrani A. Madani K. Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Separation and Purification Technology. 2016; 162: 68–76.
36. Ben Jalloul A. Chaar H. Saïdani Tounsi M. Abderrabba M. Variations in phenolic composition and antioxidant activities of Scabiosa maritima (Scabiosa atropurpurea sub. maritima L.) crude extracts and fractions according to growth stage and plant part. South African Journal of Botany. 2022; 146:703-714.
37. Kobus-Cisowska J. Szczepaniak O. Szymanowska-Powałowska D. Piechocka J. Szulc P. Dziedziński M. Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition. Ciência Rural, Santa Maria. 2020; (50)1: e20190371.
38. Sunila AV. Murugan K. Variation in phenolics, flavonoids at different stages of fruit development of Pouteria campechiana (Kunth) baehni and its antioxidant activity. International Journal of Pharmacy and Pharmaceutical Sciences. 2017; 9(11): 70-75.
39. Chepel V. Lisun V. Skrypnik L. Changes in the content of some groups of phenolic compounds and biological activity of extracts of various parts of heather (Calluna vulgaris (L.) Hull) at different growth stages. Plants. 2020; 9(8): 926.
40. Barreca D. Trombetta D. SmeriglioA. Mandalari G. Romeo O. Felice MR. Gattuso G. Nabavi SM. Food flavonols: Nutraceuticals with complex health benefits and functionalities. Trends in Food Science and Technology. 2021; 117: 194–204.
41. Zhong R. Miao L. Zhang H. Tan L. Zhao Y. Tu Y. Prieto MA. Simal-Gandara J. Chen L. He C. Cao H. Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages. Current Research in Food Science. 2020; 5: 1176–1184.
42. Brasileiro BG. Leite JPV. Casali VWD. Pizziolo VR. Coelho OGL. The influence of planting and harvesting times on the total phenolic content and antioxidant activity of Talinum triangulare (Jacq.) Willd. Acta Scientiarum. Agronomy. 2015; 37(2): 249-255.
43. de Freitas Laiber Pascoal G. de Almeida Sousa Cruz MA. Pimentel de Abreu J. Santos MCB. Bernardes Fanaro G. Júnior MRM. Freitas Silva O. Moreira RFA. Cameron LC. Simões Larraz Ferreira M. Teodoro AJ. Evaluation of the antioxidant capacity, volatile composition and phenolic content of hybrid Vitis vinifera L. varieties sweet sapphire and sweet surprise. Food Chemistry. 2022; 366:130644.
44. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181: 1199–1200.
45. Belfar A. Bensaci C. Belguidoum M. Evaluation of antioxidant capacity by cyclic voltammetry of Phoenix dactylifera L. (date palm). Asian Journal of Research in Chemistry. 2022; 15(2):138-4.
46. Ghimire BK. Seo JW. Kim SH. Ghimire B. Lee JG. Yu CW. Chung IM. Influence of harvesting time on phenolic and mineral profiles and their association with the antioxidant and cytotoxic effects of Atractylodes japonica Koidz. Agronomy. 2021; 11: 1327.
47. Makarova K. Sajkowska-Kozielewicz JJ. Zawada K. Olchowik-Grabarek E. Ciach MA, Gogolewski K. Dobros N. Ciechowicz P. Freichels H. Gambin A. Harvest time afects antioxidant capacity, total polyphenol and favonoid content of Polish St John’s wort’s (Hypericum perforatum L.) fowers. Scientifc Reports, (2021) 11:3989.
48. Ribeiro DA. Camilo CJ. de Fátima Alves Nonato C. Rodrigues FFG. Menezes IRA. Ribeiro-Filho J. Xiao J. de Almeida Souza MM. da Costa JGM. Influence of seasonal variation on phenolic content and in vitro antioxidant activity of Secondatia floribunda A. DC. (Apocynaceae). Food Chemistry. 2020; (15)315:126277.
49. Pandey MM. Khatoon S. Rastogi S. Rawat AK. Determination of flavonoids, polyphenols and antioxidant activity of Tephrosia purpurea: a seasonal study. Journal of Integrative Medicine. 2016; 14(6):447-455.
50. Do QD. Angkawijaya AE. Tran-Nguyen PL. Huynh LH. Soetaredjo FE. Ismadji S. Ju Y- H. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis. 2014; 22(3): 296-302.
51. Rafińska K. Pomastowski P. Rudnicka J. Krakowska A. Maruśka A. Narkute M. Buszewski B. Effect of solvent and extraction technique on composition and biological activity of Lepidium sativum extracts. Food Chemistry. 2019; 15(289): 16-25.
52. Sepahpour S. Selamat J. Abdul Manap MY. Khatib A. Abdull Razis AF. Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems. Molecules. 2018; 23(2): 402.
53. Huyut Z. Beydemir Ş. Gülçin İ. Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochemistry Research International. 2017; 2017:7616791.
54. Djemoui A. Djemoui D. Souli L. Souadia A. Gouamid M. The Antidiabetic, Antioxidant properties in vitro of Moringa oleifera flowers extracts grown in Sahara of Algeria. Asian Journal of Research in Chemistry. 2021; 14(3):173-8.
55. Khan RA. Evaluation of flavonoids and diverse antioxidant activities of Sonchus arvensis. Chemistry Central Journal. 2012, 6(1):126.
56. Medini F. Fellah H. Ksouri R. Abdelly C. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science. 2014; 8(3): 216-224.
57. del Baño MJ. Lorente J. Castillo J. Benavente-García O. del Río JA. Ortuño A. Quirin KW. Gerard D. Phenolic diterpenes, flavones, and rosmarinic acid distribution during the development of leaves, flowers, stems and roots of Rosmarinus officinalis. antioxidant activity. Journal of Agricultural and Food Chemistry. 2003; 51(15):4247-53.
58. Amidi S. Mojab F. Bayandori Moghaddam A. Tabib K. Kobarfard F. A simple electrochemical method for the rapid estimation of antioxidant potentials of some selected medicinal plants. Iranian Journal of Pharmaceutical Research. 2012; 11(1):117-21.
59. Magalhães LM. Segundo MA. Reis S. Lima JL. Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta. 2008; 613(1):1-19.