ABSTRACT:
Simple sensitive and accurate ion pair complex formation with reactive dyes, extractive spectrophotometric methods have developed for the estimation of clopidogrel bisulphate in pharmaceutical dosage form. The methods are based on the formation of ion paired coloured complexes by the drug with reactive dyes like Congo red, eriochrome black T and methyl orange in acidic medium. The ion associated complexes were formed and quantitatively extracted under the experimental condition in chloroform. The absorbance values were measured at 490nm, 500nm, and 430nm respectively. The proposed methods were validated statistically. Recoveries of methods were carried out by standard addition method. The linearity was found to be 5.0-30.0µg/ml, 2 -14 µg/ml, and 2-18µg/ml for methods respectively. The low values of standard deviation and percentage RSD indicate high precision of methods. Hence these methods are useful for routine estimation of emtricitabine in pharmaceutical dosages.
Cite this article:
Rajan V. Rele. Simple Extractive Spectrophotometric Method for Determination of Clopidogrel Bisulphate by Reactive dyes from Pharmaceutical Dosage Form. Asian Journal of Research in Chemistry.2026; 19(2):113-5. doi: 10.52711/0974-4150.2026.00019
Cite(Electronic):
Rajan V. Rele. Simple Extractive Spectrophotometric Method for Determination of Clopidogrel Bisulphate by Reactive dyes from Pharmaceutical Dosage Form. Asian Journal of Research in Chemistry.2026; 19(2):113-5. doi: 10.52711/0974-4150.2026.00019 Available on: https://ajrconline.org/AbstractView.aspx?PID=2026-19-2-6
REFERENCES:
1. Mohamad Ammar; Al‐Khayat; Samer Haidar; Huda Mando. Development and validation of RP‐HPLC method for determination of clopidogrel in tablets. Int. J. Pharm. Sci. Rev. Res. 2012; 14(2): 1‐5.
2. Nawal A. Alarfaj, Stability-indicating liquid chromatography for determination of clopidogrel bisulfate in tablets: Application to content uniformity testing Journal of Saudi Chemical Society. 2012; 16:23-30.
3. U.C. Mashelkar, Sanjay D. Renapurkar. A LCMS Compatible Stability-Indicating HPLC Assay Method for Clopidogrel bisulphate. International Journal of ChemTech Research. 2010; 2(2): 822-829.
4. Ajay Kumar Gupta; P.K. Patel. Analytical Method Validation of Stability Indicating HPLC Method for Determination of Assay of Clopidogrel Bisulphate Tablets USP by HPLC method. International Journal of Scientific Research. 2014; 3(2): 59-60.
5. A. Mounika; N. Sriram. Method development and validation of clopidogrel bisulphate RP_HPLC in bulk and pharmaceutical dosage form. International Journal of Pharmacy and Analytical Research. 2012; 1(1): 1-7.
6. B. Anupama; V. Jagathi; A. Aparna; M. Madhubabu; V. Lakshmi Annapurna. Assay of clopidogrel by visible spectrophotometry. International Journal of Pharma and Bio Sciences. 2011; 2(2): 105-108.
7. Chaudhari Pritam B.; Pawar Pravin D.; Narkhede Kiran P. Assay of clopidogrel by visible spectrophotometry. International Journal of Research in Ayurveda and Pharmacy. 2010; 1(2): 418-423.
8. Saadet Dermiş; Ekrem Aydoğan. Rapid and accurate determination of clopidogrel in tablets by using spectrophotometric and chromatographic techniques. Commun. Fac. Sci. Univ. Ank. Series B. 2009; 55(1): 1-16.
9. Pravin B. Cholke; Raihan Ahmed; S. Z. Chemate ; K. R. Jadhav. Development and Validation of Spectrophotometric Method for Clopidogrel bisulfate in pure and in film coated tablet dosage form Archives of Applied Science Research. 2012; 4 (1): 59-64.
10. Suhas Gurav, R.; Venkatamahesh. Development and Validation of Derivative Spectrophotometric Methods for Quantitative Estimation of Clopidogrel in Bulk and Pharmaceutical Dosage Form. International Journal of ChemTech Research. 2012; 4(2): 497-501.
11. Rajan V. Rele. Development and validation of second order derivative methods For Quantitative Estimation of Clopidogrel in bulk and Pharmaceutical Dosage Form. Der Pharmacia Lettre. 2014; 6(6):117-121.
12. D. Antić; S. Filipić; D. Agbaba. A simple and sensitive TLC method for determination of clopidogrel and its impurity SR 26334 in pharmaceutical products. Acta Chromatographica. 2007; 18: 199-206.