ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Atul H Kategaonkar, Dhaval M Patel, Bhushan P Itkar, Vishnu P Choudhari, Bhanudas S Kuchekar, Ana G Nikalje

Email(s): viraj1404@rediffmail.com

DOI: Not Available

Address: Atul H Kategaonkar1, Dhaval M Patel1, Bhushan P Itkar1, Vishnu P Choudhari1*, Bhanudas S Kuchekar1 and Ana G Nikalje2
1MAEER’S Maharashtra Institute of Pharmacy, S.No. 124, MIT Campus, Paud Road, Kothrud, Pune-411038, India
2Post-graduate Department of Pharmaceutical Chemistry, Y.B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad.431001. MS. India
*Corresponding Author

Published In:   Volume - 2,      Issue - 4,     Year - 2009


ABSTRACT:
Simple, accurate, precise, and sensitive ultraviolet spectrophotometric and stability indicating reversed phase high performance liquid chromatographic methods for simultaneous estimation of Tamsulosin and Finasteride in combined tablet dosage form have been developed and validated. The spectroscopic method employs an absorbance correction method using 228 and 246 nm as two wavelengths for estimation with methanol as solvent. Beer's law is obeyed in the concentration range of 2-10 and 25-125 µg/mL for Tamsulosin and Finasteride respectively. The reversed phase high performance liquid chromatographic separation was achieved on a Waters Symmetry C18 column (250 mm x 4.6 mm, 5.0 µ particle size) using Methanol: Water: THF (75: 15: 10 v/v/v) mobile phase. Trifluoroacetic was used to adjusted pH to 3.7 with, flow rate was 0.7 mL/min and column temperature was maintained at 320C. Quantification was achieved with PDA detector at 275 nm over the concentration range of 50 to 400µg/mL for Finasteride and 4 to 44µg/mL for Tamsulosin. Both methods have been successfully applied for the analysis of the drugs in a pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.


Cite this article:
Atul H Kategaonkar, Dhaval M Patel, Bhushan P Itkar, Vishnu P Choudhari, Bhanudas S Kuchekar, Ana G Nikalje. Spectrophotometric and Stability Indicating RP-HPLC-PDA method for Simultaneous Determination of Finasteride and Tamsulosin in Combined Tablet Dosage form. Asian J. Research Chem. 2(4):Oct.-Dec. 2009 page 414-419.


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