ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Sachin Patil, B Ramesh, AR Hareesh, Kiran Patil, Ashok Dhokane

Email(s): patilsirwarana@yahoo.com

DOI: Not Available

Address: Sachin Patil*1, B Ramesh1, AR Hareesh1, Kiran Patil2 and Ashok Dhokane2
1Shri Adichunchunagiri College of Pharmacy, B. G. Nagara, Tal: Nagmangala Dist: Mandya, Karnataka, India
2Government College of Pharmacy, Vidyanagar, Karad, Dist-Satara, Maharashtra-415 124, India
*Corresponding Author

Published In:   Volume - 3,      Issue - 3,     Year - 2010


ABSTRACT:
Simple, precise, sensitive and reproducible quantitative reverse phase HPLC method has been developed for estimation of Sitagliptin phosphate in pharmaceutical dosage form. The method was carried out on a Phenomenex RP -18 (2) Column (250×4.6mm i.d; particle size 5µm) column with a mobile phase consisting of (60:40 v/v Acetonitrile: water) at a flow rate of 1.0 ml/min. The eluents were monitored at 272 nm. The retention time of Sitagliptin phosphate was found out to be 5.062. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantification. The proposed method can be used for the estimation of this drug in pharmaceutical dosage forms.


Cite this article:
Sachin Patil, B Ramesh, AR Hareesh, Kiran Patil, Ashok Dhokane. Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 653-655.


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