Firoj A. Tamboli, Harinath N. More, Susmita S. Bhandugare, Ankita S. Patil, Namdeo R. Jadhav, Suresh G. Killedar
Firoj A. Tamboli1*, Harinath N. More1, Susmita S. Bhandugare1, Ankita S. Patil1, Namdeo R. Jadhav1, Suresh G. Killedar2
1Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Near chitranagari, Kolhapur -416013 Maharashtra, India.
2Sant Gajanan Maharaj College of Pharmacy, Mahagaon Tal- Gadhinglaj Dist- Kolhapur 416503 Maharashtra, India.
Volume - 13,
Issue - 5,
Year - 2020
Eichhornia crassipes is free-floating aquatic macrophytes. For performing normal body function carbohydrate is an essential source for living organisms. Low carbohydrate intake results in muscle breakdown, ketosis, and dehydration. Daily 50-100gms of carbohydrate is recommended. In this study, the total carbohydrate content was detected in the aqueous extract by using phenol- sulphuric acid method. This was a rapid colorimetric method that determines the total carbohydrate present in the sample. The role of sulphuric acid is to convert polysaccharides, oligosaccharides, and disaccharides into monosaccharides. The result showed that the carbohydrate content was 30.50% in the sample solution. So the weed can be utilized as the source of carbohydrate.
Cite this article:
Firoj A. Tamboli, Harinath N. More, Susmita S. Bhandugare, Ankita S. Patil, Namdeo R. Jadhav, Suresh G. Killedar. Estimation of Total Carbohydrate content by Phenol Sulphuric acid Method from Eichhornia crassipes (Mart.) Solms. Asian J. Research Chem. 2020; 13(5):357-359. doi: 10.5958/0974-4150.2020.00067.X
1. Omondi, E.A., Ndiba, P.K. & Njuru, P.G. Characterization of water hyacinth (E. crassipes) from Lake Victoria and ruminal slaughterhouse waste as co-substrates in biogas production. SN Appl. Sci. 2019; 1, 848.
2. Tao R, Zeng R, Xiao-Yan Y, Zhang S, Yang ZH. Water Hyacinth (Eichhornia crassipes) Biomass as a Biofuel Feedstock by Enzymatic Hydrolysis. Bio Resources.2016; 11(1):2372–2380.
3. Degaga AH. Water Hyacinth (Eichhornia crassipes) Biology and its Impacts on Ecosystem, Biodiversity, Economy and Human Wellbeing. J. Life Sci. Biomed. 2018;8(6): 94-100.
4. BVVS Surya Kiran, Bishnupada Biswal, G Nagarjuna Reddy, LK Kanthal, N Sridhar. Evaluation of anthelmintic activity of Eichhornia crassipes roots. Research J. Pharmacology and Pharmacodynamics. 2013; 5(3): 183-184.
5. Ali H. et al. The world's worst aquatic plant as a safe cancer medicine Antitumor activity on melanoma induced mouse by Eichhornia crassipes: in vivo studies. J Pharm Res. 2009; 2(1):365-6.
6. Shanab S.M. et al. Allelopathic Effects of water hyacinth (Eichhornia crassipes). PlusOne. 2010; 5:1-8.
7. Owens, C.S and Madsen, J.D. Low temperature limits of waterhyacinth. Journal of Aquatic Plant Management. 1995; 33: 63– 68.
8. B.S. Krishnamoorthy, N. Nattuthurai, Arunkumar K., Revathi T., S. Kamatchi. Preliminary Studies on Phytochemicals of Solvent Extracts of Eichhornia crassipes. Research J. Pharm. and Tech. .2014; 7(6): 635-637.
9. Navarro L and Phiri G. Water hyacinth in Africa and the Middle East. A survey of problems and solutions. International Development Research Centre, Ottawa (CA). 2000.
10. Hammuel C, Anyim B P, Raplong H H, Inekwe U V, Batari M L. Phytochemical Screening and In Vitro Antibacterial Acitivity of Methanol and Aqueous Extracts of Acalypha Racemosa Leaves. Res. J. Pharmacognosy & Phytochem. 2014; 6(3): 118-121.
11. Praveen Kumar Uppala, K. Atchuta Kumar, Sujit Kumar Patro, Murali Krishna. B. Experimental Evaluation of Antidepressant activity of Aqueous and Chloroform Leaf and Shoot Extracts of Eicchornia crassipes Linn in Mice. Asian J. Res. Pharm. Sci. 2015; 5(3): 139-144.
12. Madsen JD. Seasonal biomass and carbohydrate allocation in a southern population of Eurasian watermilfoil. J Aquat Plant Manage. 1997; 35:15–21.
13. Nielson S.S.: Phenol-Sulfuric Acid method for total carbohydrates. Food Science Text Series. 2010; pp.47-53.
14. Quantitative Evaluation of Carbohydrate Levels in Different Natural Foodstuffs by UV-Visible Spectrophometer. Asian J. Pharm. Ana. 2012; 2(1): 10-11.
15. V.S. Asha, Ch. Madhu, Kavya. Mannem, V. Prateesh Kumar. Quantitative Evaluation of Carbohydrate Levels in Seeds for Home Use by UV-Visible Spectrophotometer. Asian J. Pharm. Ana. 2012; 2(3):71-72.
16. Kavya Mannem, Ch. Madhu, V.S. Asha, V. Prateesh Kumar. Quantitative Evaluation of Carbohydrate Levels in Green Leafy Vegetables for Home use by UV-Visible Spectrophotometer. Asian J. Pharm. Ana.2012; 2(3): 79-80.
17. Ch. Madhu, J. Swapna, R. Sneha Spandana, I. Niharika, K. Rohith Raj, B. Kalyan, M. Raja Rajeshwari, T. Prathyusha. Quantitative Evaluation of Carbohydrate Levels in Seeds for Home use By UV-Visible Spectrophotometer. Asian J. Pharm. Ana. 2012; 2(4): 104-105.
18. Vavilala Prateesh Kumar, Ch. Madhu, Kavya Mannem, V.S. Asha, A. Sambasiva Rao, M. Sravan Prasad. Quantitative Evaluation of Carbohydrate Levels in Fruits by UV-Visible Spectrophotometer. Asian J. Pharm. Tech. 2012; 2(3): 99-100.
19. Ch. Madhu, J. Swapna, R. Sneha Spandana, I. Niharika, K. Rohith Raj, B. Kalyan, M. Rajarajeshwari, T. Prathyusha. Quantitative Evaluation of Carbohydrate Levels in Seeds for Home use by UV-Visible Spectrophotometer. Asian J. Pharm. Tech. 2012; 2(3): 110-111.
20. Bokov D.O., Kulaeva I.R., Potanina O.G., Sergunova E.V., Bondar A.A., Evgrafov A.A., Antsyshkina A.M., Krasnyuk I.I.. Carbohydrates determination in the Snowdrops (Galanthus L.) herbal pharmaceutical substances by TLC and UV-Spectrophotometry. Research J. Pharm. and Tech. 2020; 13(1):243-249.