ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Rele R.V.., Warkar C.B.

Email(s): warkarchandan@rediffmail.com

DOI: Not Available

Address: Rele R.V.* and Warkar C.B.
D. G. Ruparel College, Matunga, Mumbai 400 016
*Corresponding Author

Published In:   Volume - 3,      Issue - 3,     Year - 2010


ABSTRACT:
A gradient reverse phase HPLC method was developed and validated for simultaneous estimation of ciprofloxacin hydrochloride and dexamethasone in pharmaceutical dosage form i.e. ophthalmic solution using Nucleosil C18 (15cm x 4.6mm, 5µm) column with a flow rate of 1.2 ml/minute and detection of 265nm. The mobile phase A consisted of 50 milli molar citric acid and potassium phosphate buffer. The mobile phase B consisted of 100 % v/v acetonitrile. The method was validated for system suitability, linearity, accuracy and precision. The system suitability parameters like resolution, theoretical plates and tailing factor were evaluated. The linear range for ciprofloxacin was 3 µg/ml – 55 µg/ml and for dexamethasone was 1 µg/ml – 16 µg/ml. The method has been successfully used to analyze pharmaceutical dosage form i.e. ophthalmic solution containing ciprofloxacin hydrochloride and dexamethasone with good recoveries.


Cite this article:
Rele R.V.., Warkar C.B.. Simultaneous Determination of Ciprofloxacin Hydrochloride and Dexamethasone in Ophthalmic Solution by Reversed Phase High Performance Liquid Chromatography. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 673-677


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