ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): D.D. Chougule, N.S. Naikwade

Email(s): dr_naikwade@rediffmail.com

DOI: Not Available

Address: D.D. Chougule* and N.S. Naikwade
Appasaheb Birnale College of Pharmacy, Sangli. South-Shivaji Nagar, Sangli 416416 (MS)
*Corresponding Author

Published In:   Volume - 4,      Issue - 1,     Year - 2011


ABSTRACT:
A simple, selective, rapid and precise reverse phase HPLC method has been developed for the estimation of Deflazacort in pharmaceutical dosage form. A Hypersil BDS C18 (250 mm X 4.6mm) column was used for separation. The mobile phase was ammonium acetate buffer solution: acetonitrile (50:50 v/v). Flow rate 1.0ml/min with detection at 254 nm. The retention time of Deflazacort was 6.03min. Linearity obtained in the concentration range of 20-100µg/ml with correlation coefficient of 0.998.The result of the analysis were validated statistically and recovery studies confirmed by the accuracy of the proposed method. The proposed RP-HPLC method for the estimation of Deflazacort in dosage form is accurate, precise, linear, robust, simple, rapid and selective.


Cite this article:
D.D. Chougule, N.S. Naikwade. Development and Validation of High Performance Liquid Chromatographic Method for Estimation of Deflazacort in Pharmaceutical Formulation. Asian J. Research Chem. 4(1): January 2011; Page 140-142.


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