ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Govind Sharma, Nitin Bansal, Dinesh Kumar Jain, Shekhar Verma, A. K. Jha

Email(s): govindsharma10aug@gmail.com

DOI: Not Available

Address: Govind Sharma1*, Nitin Bansal2 , Dinesh Kumar Jain2, Shekhar Verma1and A. K. Jha1
1Faculty of Pharmaceutical Sciences, SSTC, Junwani, Bhilai (CG)
2College of Pharmacy, IPS Academy, Indore [Madhya Pradesh]-452012, India
*Corresponding Author

Published In:   Volume - 5,      Issue - 7,     Year - 2012


ABSTRACT:
Simple, accurate, precise, and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Paracetamol (PCM), Tramadol and Aceclofenac in the presence of its degradation products, have been developed and validated by LC/MS. Linearity of PCM, TRA and ACL was obeyed in concentration range 2-30, 2-14 and 2–35 µg/ml in Acetonitrile: Phosphate buffer (pH 3.4, 20:80 v/v) as the mobile phase at 270 nm, respectively. The detection was carried out using a diode array detector set at 270 nm. Linearity of the LC method was in the concentration range of 2-10 µg/mL for PCM, TRA and ACL respectively. The recoveries were in the range of 99.49 ± 0.74 to 100.08 ± 0.1 for PCM, 99.36 ± 0.73 to 100.05 ± 0.10 for TRA and 98.42 ± 0.96 to 101.04 ± 0.49 for ACL in RP-HPLC. RP-HPLC method has been successfully applied for the analysis of the drugs in a pharmaceutical formulation in the presence of its degradation products. Results of analysis were validated statistically. Degradation study was performed under different conditions using same RP-HPLC method and method was revalidated for simultaneous estimation in presence of their degradation products and confirmed by LC/MS.


Cite this article:
Govind Sharma, Nitin Bansal, Dinesh Kumar Jain, Shekhar Verma, A. K. Jha. Reversed-Phase High-Performance Liquid Chromatographic and Mass . Spectrophotometric Methods for Simultaneous Determination of Paracetamol, Aceclofenac and Tramadol in Combined Tablet Dosage Form in Presence of its Degradation Products. Asian J. Research Chem. 5(7): July, 2012; Page 854-858.


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