Traditional system of medicine recommends various hepatoprotective agents and preparations to treat hepatic disorders. Polyherbal commercial extracts formulations (tablet) was developed by using well documented medicinal plants, Cassia fistula, Coccina indica and Vigna mungo for treatment of liver disorders by exploiting the knowledge of Traditional system of medicine. The stationary phase was precoated aluminium silica gel G F 254 Plates. The mobile phase for aspartic acid and vitexin were n-Butenol: Glacial acetic Acid: Water (50: 10: 40) and Ethyl acetate: Formic acid: Glacial acetic Acid: Water (100: 11: 11: 26) respectively. The plate was scanned and quantified at 544 nm for aspartic acid and 366 nm for vitexin. The amount of aspartic acid and vitexin were estimated by the comparing the peak area of standard and the same were present in the tablet formulation. The content of aspartic acid and vitexin were found to be 0.2624% w/w and 0.1239% w/w in polyherbal commercial extracts tablet formulation respectively. In order to obtain precission and accuracy the recovery study were performed and result obtained with mean value 99.89 % and 101.44 % for aspartic acid and vitexin respectively, which prove reproducibility of the result. The mean of % RSD value was found to be 1.54 and 1.13 for aspartic acid and vitexin respectively. This estimation technique is very much useful for the estimation of aspartic acid and vitexin present in the various formulations.
Cite this article:
Praveen Patidar, Sameer Singh, Narendra Madoria, Kamlesh Dashora. HPTLC Estimation of Aspartic Acid and Vitexin in Polyherbal Tablet Prepared By Commercial Extracts. Asian J. Research Chem. 5(8): August, 2012; Page 1057-1060.