Author(s):
Bachir Ben Seghir, Iham Ben Amor, Ahlam Beki, Imane Souici, Hadia Hemmamia, Zeghoud Soumeia, Salah Eddine Laouini, Abdelkrim Rebiai
Email(s):
bbachir39@gmail.com
DOI:
10.5958/0974-4150.2020.00031.0 
Address:
Bachir Ben Seghir1, Iham Ben Amor2, Ahlam Beki 2, Imane Souici2, Hadia Hemmamia3,
Zeghoud Soumeia3, Salah Eddine Laouini2, Abdelkrim Rebiai3
1Laboratory of Industrials Analysis and Materials Engineering, University 8 May 1945 Guelma,
BP 401 Guelma 24000, Algeria.
2Department of Process Engineering, Faculty of Technology, University Hamma Lakhdar El Oued,
BP 789, El-Oued39000, Algeria.
3Laboratory Valorisation and Technology of Saharan Resources (VTRS). University of El Oued,
BP 789 El-Oued (39000) Algeria.
*Corresponding Author
Published In:
Volume - 13,
Issue - 3,
Year - 2020
ABSTRACT:
The wool consists of around 90 % keratin, an essential ingredient in cosmetics, shampoos, and hair care creams. The goal of this study was to determine the optimum conditions for keratin from wool extraction. Keratin isolation using different agents for sulfitolysis has been studied using statistical experimental method. In the extraction procedure, concentration Thiourea (CH4N2S), temperature and time reaction were analysed. The optimization yield (%) of keratin was achieved at obtained corresponds to the concentration Thiourea of x1= 8.6 %, the tempera-ture of x2= 65.50 °C and the reaction time of x3= 2 h, using response surface methodology (RSM). In addition, because of its good the model of experiment was significant with p<0.05 and adj R2 = 0.9991 with a good yield of keratin.
Cite this article:
Bachir Ben Seghir, Iham Ben Amor, Ahlam Beki, Imane Souici, Hadia Hemmamia, Zeghoud Soumeia, Salah Eddine Laouini, Abdelkrim Rebiai. Preparation and Optimization of Keratin from Sheep wool using response Surface Methodology. Asian J. Research Chem. 2020; 13(3):157-162. doi: 10.5958/0974-4150.2020.00031.0
Cite(Electronic):
Bachir Ben Seghir, Iham Ben Amor, Ahlam Beki, Imane Souici, Hadia Hemmamia, Zeghoud Soumeia, Salah Eddine Laouini, Abdelkrim Rebiai. Preparation and Optimization of Keratin from Sheep wool using response Surface Methodology. Asian J. Research Chem. 2020; 13(3):157-162. doi: 10.5958/0974-4150.2020.00031.0 Available on: https://ajrconline.org/AbstractView.aspx?PID=2020-13-3-1
REFERENCES:
1.
Zhang Y, Yang R, Zhao W. Improving digestibility
of feather meal by steam flash explosion. J Journal of Agricultural Food
Chemistry. 2014;62(13):2745-2751.
2.
Dou
Y, Huang X, Zhang B, He M, Yin G, Cui Y. Preparation and characterization of a
dialdehyde starch crosslinked feather keratin film for food packaging application.
J Rsc Advances. 2015;5(34):27168-27174.
3.
Kamarudin
NB, Sharma S, Gupta A, Kee CG, Chik SMSBT, Gupta R. Statistical investigation
of extraction parameters of keratin from chicken feather using Design-Expert. J
3 Biotech. 2017;7(2):127.
4.
Shavandi
A, Silva TH, Bekhit AA, Bekhit AE-DA. Keratin: dissolution, extraction and
biomedical application. J Biomaterials Science. 2017;5(9):1699-1735.
5.
Kushwaha
RK, Guarro J. Biology of dermatophytes and other keratinophilic fungi: Revista
Iberoamericana de Micologia; 2000.
6.
Kumar
SL, Anandhavelu S, Sivaraman J, Swathy M. Modified extraction and
characterization of keratin from Indian goat hoof: A biocompatible biomaterial
for tissue regenerative applications. J Integrated Ferroelectrics 2017;184(1):41-49.
7.
Sharma
S, Gupta A, Chik S, Gek K, Podde P, Thraisingam J, Subramaniam M. Extraction
and characterization of keratin from chicken feather waste biomass: a study.
In.Proceedings of the national conference for postgraduate research (NCON-PGR
2016), Universiti Malaysia Pahang (UMP), Pekan; 2016. p. 693-699.
8.
Shavandi
A, Carne A, Bekhit AA, Bekhit AE-DA. Conversion of waste wool to protein using
a simple oxidation method.
9.
Poole
AJ, Lyons RE, Church JS. Dissolving feather keratin using sodium sulfide for
bio-polymer applications. J Journal of Polymers the Environment.
2011;19(4):995-1004.
10.
Rajabinejad
H, Zoccola M, Patrucco A, Montarsolo A, Rovero G, Tonin C. Physicochemical
properties of keratin extracted from wool by various methods. J Textile
Research Journal. 2018;88(21):2415-2424.
11.
Teklemedhin
B. Cationization of Cotton Using Extracted Keratin from Ethiopian Sheep Wool
Waste for Salt Free Dyeing with Reactive Dye. J Textile Sci Eng. 2018;8(365):2.
12.
Shavandi
A, Bekhit AE-DA, Carne A, Bekhit A. Evaluation of keratin extraction from wool
by chemical methods for bio-polymer application. J Journal of Bioactive
Compatible Polymers. 2017;32(2):163-177.
13. District HBT.
Silver nanoparticles: synthesis, properties, toxicology, applications and
perspectives. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2013;4(3):033-001.
14.
Bezerra MA, Santelli RE, Oliveira
EP, Villar LS, Escaleira LA. Response surface methodology (RSM) as a tool for
optimization in analytical chemistry. J Talanta. 2008;76(5):965-977.
15.
Seghir
BB, Benhamza M. Preparation, optimization and characterization of chitosan
polymer from shrimp shells. J Journal of Food Measurement Characterization.
2017;11(3):1137-1147.
16.
Yeul
VS, Rayalu SS. Unprecedented chitin and chitosan: A chemical overview. Journal
of Polymers the Environment. 2013;21(2):606-614.
17.
Benhabiles
M, Salah R, Lounici H, Drouiche N, Goosen M, Mameri N. Antibacterial activity
of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food Hydrocolloids.
2012;29(1):48-56.