Atorvastatin Calcium and Ramipril are used in combination for treatment of hypertension. The present work deals with
simple spectrophotometric method development for simultaneous estimation of Atorvastatin Calcium (ATR) and Ramipril (RAM) in two component capsule formulation. The method employed first order derivative spectroscopy1. For determination of sampling wavelength 10 µ g/ml of each of ATR and RAM were scanned in 200-350 nm range and sampling wavelengths were 294 nm for ATR were RAM showed zero crossing point and 229 nm for RAM were ATR showed zero crossing point in first order derivative spectroscopy. For this method linearity observed in 5-40 µ g/ml for ATR and 2-20 µ g/ml for RAM. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines.
Cite this article:
Thamake SL, Jadhav SD, Pishawikar SA. Development and Validation of Method for Simultaneous Estimation of Atorvastatin Calcium and Ramipril from Capsule Dosage Form by First Order Derivative Spectroscopy. Asian J. Research Chem. 2(1): Jan.-March, 2009;Page 52-53.