Nidhi Dubey, Nitin Dubey, Rajendra Mehta, Ajay Saluja
Nidhi Dubey 1,*, Nitin Dubey 2, Rajendra Mehta3 and Ajay Saluja 3
1School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore [M P], India.
2College of Pharmacy, IPS Academy, Indore [M P], India
3A. R .College of Pharmacy, Vallabh vidyanagar [Gujarat], India.
* Corresponding Author
Volume - 2,
Issue - 1,
Year - 2009
A simple, sensitive, rapid and selective high-performance liquid chromatographic (HPLC) method has been developed
and validated for the analysis of catechin in marketed Ayurvedic Taila (oil) formulations containing Acacia catechu. Chromatography of methanolic: 0.1% formic acid (7:3, v/v) extracts of these formulations was performed on C18 (5 µm x
25cm x 4.6mm i.d) column using isocratic mobile phase consisting of methanol: acetonitrile: water (40:15:45, v/v/v)
containing 1.0% acetic acid at a flow rate of 0.5ml/min and SPD-10 AVP photodiode array (PDA) UV-Visible detector. The analytical marker, catechin, was quantified at 279 nm. The retention time of catechin was about 3.27 min. The linear regression analysis data for the calibration plot showed a good linear relationship with correlation coefficient of 0.9988 in the concentration range of 15 to 90 µ g/ml for catechin with respect to peak area. The limit of detection and limit of quantitation values were found to be 0.5µ g/ml and 1.7µ g/ml respectively. Repeatability of the method was found to be
0.62 RSD. Recovery values from 99.73 to 100.22 % indicate excellent accuracy of the method. The developed HPLC method is accurate, precise, and cost-effective, and it can be successfully applied for the determination of catechin in marketed ayurvedic oil formulations containing Acacia catechu.
Cite this article:
Nidhi Dubey, Nitin Dubey, Rajendra Mehta, Ajay Saluja. A Selective High Performance Liquid Chromatographic Method for Estimation of Catechin in Ayurvedic Taila Preparations. Asian J. Research Chem. 2(1): Jan.-March, 2009;Page 66-69.