K Ananda kumar, G Kumaraswamy, T Ayyappan, ASK Sankar, D Nagavalli
K Anandakumar*, G Kumaraswamy, T Ayyappan, ASK Sankar and D Nagavalli
Adhiparasakthi College of Pharmacy, Melmaruvathur - 603 319, Tamil Nadu, India
Volume - 3,
Issue - 1,
Year - 2010
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Eszopiclone (ESZ) in bulk and in tablet dosage form. Isocratic elution at a flow rate of 1ml/ min was employed on a Phenomenax Luna C18 column (150 ? 4.6 mm; 5?) at ambient temperature. The mobile phase consisted of Acetonitrile: phosphate buffer adjusted to pH 2.5 (25:75% v/v). The UV detection wavelength was 304 nm and 20 ?l of sample was injected. The retention time for ESZ was 3.92 min. The method obeys Beer's law in the concentration range of 4-24 ?g/ ml. The amount of Eszopiclone Present in the formulation was found to be 99.60 ? 1.5389. The % recovery was in the range between 99.25% and 99.86%. The percentage RSD for precision and accuracy of the method was found to be less than 2 %. The method was validated as per the standard analytical procedures and statistical parameters. The method was successfully applied for routine analysis of ESZ in bulk and in formulation.
Cite this article:
K Ananda kumar, G Kumaraswamy, T Ayyappan, ASK Sankar, D Nagavalli. Validated RP-HPLC Method for the Estimation of Eszopiclone in Bulk and Tablet Dosage Form. Asian J. Research Chem. 3(1): Jan.-Mar. 2010; Page 63-66.