A Simple, accurate and precise method for simultaneous estimation of Tramadol Hydrochloride and Paracetamol in their combined dosage form have been described. The spectrophotometric method is based on zero absorbance measurement, which uses 272 and 250 nm as two analytical wavelengths. Beer’s law was obeyed in the concentration range of 60 to 160 µg/ml for Tramadol hydrochloride and 8 to 30 µg/ml for Paracetamol. The LOD and LOQ were found to be 25 µg/ml and 60 µg/ml for Tramadol hydrochloride and 3µg/ml and 8 µg/ml for Paracetamol, respectively. The results obtained in the analysis of dosage form by developed method are well agreed with the labeled contents.
Cite this article:
Paresh B. Shah, Boni B. Shah. Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Tramadol Hydrochloride and Paracetamol from their Combined Dosage Form. Asian J. Research Chem. 3(1): Jan.-Mar. 2010; Page 17-19.