A simple, specific, accurate and precise high performance liquid chromatographic method was developed for the determination of Amlodipine and Atorvastatin in Amlodipine besylate and Atorvastatin tablet dosage forms. A Hypersil BDS C18 5µm (250x4.6mm I.D) with column temperature 30°C in isocratic mode, with mobile phase containing Acetonitrile: 10mM Ammonium acetate buffer pH 4.0 (50:50) was used. The flow rate was 1.0mL/min and effluent was monitored at 238nm.The retention time of Amlodipine was 2.5min and for Atorvastatin was 4.7min.The linearity for Amlodipine and Atorvastatin was in the range 0.02mg/mL to 0.12mg/mL.The proposed method is accurate, precise, specific and rapid estimation of Amlodipine and Atorvastatin in Amlodipine besilate and Atorvastatin tablets.
Cite this article:
Ravinder Burugu, G. Venkateshwarlu. RP-HPLC Determination of Amlodipine Besylate and Atorvastatin from Tablets Dosage forms. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 763-766.