Parag R. Patel, Vandana B. Patel, Mayank Bapna
Parag R. Patel1, Vandana B. Patel2* and Mayank Bapna2
1Pharmaceutical Quality Assurance Laboratory, Pharmacy Department, Faculty of Technology and Engineering, The Maharaja Sayajirao University of Baroda, Vadodara 390 001, Gujarat (India)
2 Baroda College of Pharmacy, P.O. Limda, Ta. Waghodia, Dist. Vadodara, Pin 391 760 Gujarat (India)
Volume - 3,
Issue - 4,
Year - 2010
Rapid, precise, accurate and specific ratiospectra derivative spectrophotometry and high-performance liquid chromatography procedures were described for the simultaneous determination of levofloxacin and ornidazole in their combined dosage forms. For the first method of ratiospectra derivative spectrophotometry the signals were measured at 302 nm for ornidazole and 292.8 nm for levofloxacin as a mixture. The second method is based on high performance liquid chromatography (HPLC) on Princeton RP-C18 column (5µ, 150x4.6mm) using 0.1% v/v triethylamine-acetonitrile (80:20, v/v) (pH 6.0). Detection was carried out using a UV detector at 295 nm. The retention time for levofloxacin and ornidazole were found to be 2.8 min and 4.4 min respectively. Both the methods showed good linearity in the range of 1 to 20µg/mL and 5 to 5000ng/ml for ratiospectra derivative spectroscopy and HPLC respectively.
Pharmaceutical formulations containing both drugs were successfully assayed using the developed methods.
Cite this article:
Parag R. Patel, Vandana B. Patel, Mayank Bapna. Simultaneous Determination of Levofloxacin and Ornidazole by Ratiospectra Derivative Spectrophotometry and High Performance Liquid Chromatography. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 922-927.