D. Boopathy, Devaki Magham, B. Arun Kumar, V. Sekar, P. Perumal
D. Boopathy*, Devaki Magham, B. Arun Kumar, V. Sekar and P. Perumal
Department of Pharmaceutical Analysis, J.K.K. Nattraja College of Pharmacy, Komarapalayam -638 183, Namakkal (DT), Tamil Nadu, India
Volume - 4,
Issue - 7,
Year - 2011
A Simple and reproducible method for simultaneous estimation of Diethylcarbamazine citrate (DECC) and Cetirizine hydrochloride (CTH) by High performance liquid chromatography (HPLC) in formulation was developed. The analysis was performed with a mobile phase containing Acetonitrile and mixed phosphate buffer (KH2PO4 and K2HPO4, adjusted to pH – 3.0 with 10% orthophosphoric acid) in the ratio of 60:40(%v/v) at a flow rate of 1ml/min. A phenomenex C18 column (250 × 4.6mm,5µm)was used. The wavelength of DECC is 216nm and Cetirizine is 230nm and from the overlapping spectra a wavelength of 220nm is selected and simultaneous estimation is carried out .The analysis was performed in the linearity range of 24-144 µg/ml for Diethylcarbamazine citrate and 0.8 - 4.8 µg/ml for Cetirizine hydrochloride and the minimum detectable level was 0.62 µg/ml and 0.105 µg/ml.The correlation coefficient was 0.998 and 0.997.Thus the developed and validated chromatographic method for Diethylcarbamazine citrate and Cetirizine hydrochloride is said to be rapid, simple, accurate and cost effective that can be applied for routine analysis in research institution, quality control department in industries and in clinical pharmacokinetic studies.
Cite this article:
D. Boopathy, Devaki Magham, B. Arun Kumar, V. Sekar, P. Perumal. Simultaneous Estimation of Diethylcarbamazine Citrate and Cetirizine Hydrochloride in Formulation by RP-HPLC. Asian J. Research Chem. 4(7): July, 2011; Page 1064-1066.