Author(s):
Saroj Kumar Raul, B.V.V Ravi Kumar, Ajaya Kumar Patnaik, Nagireddy Neelakanta Rao
Email(s):
saroj.raul@rediffmail.com
DOI:
Not Available
Address:
Saroj Kumar Raul1*, B.V.V Ravi Kumar2, Ajaya Kumar Patnaik3, Nagireddy Neelakanta Rao4
1M .R. College of Pharmacy, Phool-Baugh, Vizianagaram, A.P, India.
2Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India.
3Departments of Chemistry, Ravenshaw University, Cuttack, Orissa, India.
4Suraksha Pharma Ltd, Suraksh Towers, Hyderabad, A.P, India.
*Corresponding Author
Published In:
Volume - 5,
Issue - 11,
Year - 2012
ABSTRACT:
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Olopatadine in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL min -1 was employed on a Kromasil C18 column at ambient temperature. The mobile phase consisted of methanol: phosphate buffer 60:40 (v/v) and the detection wavelength was at 246 nm. Linearity was observed in concentration range of 10-60 µg/mL. The retention time for Olopatadine was 2.4 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Olopatadine in pharmaceutical dosage forms.
Cite this article:
Saroj Kumar Raul, B.V.V Ravi Kumar, Ajaya Kumar Patnaik, Nagireddy Neelakanta Rao. A RP-HPLC Method Development and Validation for the Estimation of Olopatadine in Bulk and Pharmaceutical Dosage forms. Asian J. Research Chem. 5(11): Nov., 2012; Page 1395-1398.
Cite(Electronic):
Saroj Kumar Raul, B.V.V Ravi Kumar, Ajaya Kumar Patnaik, Nagireddy Neelakanta Rao. A RP-HPLC Method Development and Validation for the Estimation of Olopatadine in Bulk and Pharmaceutical Dosage forms. Asian J. Research Chem. 5(11): Nov., 2012; Page 1395-1398. Available on: https://ajrconline.org/AbstractView.aspx?PID=2012-5-11-16