ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): B. Raja, A. Lakshmana Rao

Email(s): dralrao@gmail.com

DOI: Not Available

Address: B. Raja1 and A. Lakshmana Rao2*
1Anurag Pharmacy College, Ananthagiri- 508 206, A.P., India.
2*V.V. Institute of Pharmaceutical Sciences, Gudlavalleru- 521 356, A.P., India.
*Corresponding Author

Published In:   Volume - 6,      Issue - 11,     Year - 2013


ABSTRACT:
A simple, rapid, accurate and precise isocratic reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous estimation of Ramipril and Felodipine in tablet dosage forms. The chromatographic separation was carried out on Hypersil BDS C18 column (150 x 4.6 mm; 5µ) with a mixture of phosphate buffer pH 5.5: acetonitrile (40: 60 V/V) as a mobile phase; at a flow rate of 1.0 mL/min. UV detection was performed at 243 nm. The retention times were 2.844 min and 5.727 min for Ramipril and Felodipine respectively. Calibration plots were linear (r2=1) over the concentration range of 5-30 µg/mL for both Ramipril and Felodipine. The method was validated for linearity, accuracy, precision, specificity and sensitivity. The proposed method was successfully used for quantitative analysis of Ramipril and Felodipine tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that the method is specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation confirm the suitability of the proposed method for routine estimation of Ramipril and Felodipine in bulk and tablet dosage form.


Cite this article:
B. Raja, A. Lakshmana Rao. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ramipril and Felodipine in Pharmaceutical Dosage Forms. Asian J. Research Chem. 6(11): November 2013; Page 1018-1022


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