ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): G. Saravanan, Md. Yunoos, B. Parveena

Email(s): sarachem1981@gmail.com

DOI: Not Available

Address: G. Saravanan*, Md. Yunoos, B. Parveena
Department of Pharmaceutical Chemistry, Bapatla College of Pharmacy, Bapatla-522101, Andhra Pradesh
*Corresponding Author

Published In:   Volume - 7,      Issue - 2,     Year - 2014


ABSTRACT:
A simple, rapid and sensitive isocratic RP-HPLC method was developed for the simultaneous estimation of Perindopril and Indapamide present in bulk and their pharmaceutical formulations using Thermo hypersil BDS C18 (250 × 4.6 mm i.d, packed with 5 µ particle size) column at ambient temperature in an isocratic mode with mobile phase comprising KH2PO4 buffer adjusted to pH 3.5: Methanol in the ratio of 80:20 (% v/v). The flow rate was 0.8 ml/ min and effluent was monitored at 215 nm. The retention time were found to be 3.54 min for Perindopril and 4.10 min for Indapamide. The assay exhibited a linear concentration range of 160-480 µg/ml for Perindopril and 50-150 µg/ml for Indapamide with a correlation coefficient of 0.9992 for Perindopril and 0.9999 for Indapamide respectively. Recovery was found to be 99.92 % + 0.42 for Perindopril and 99.75 % + 0.28 for Indapamide respectively. The proposed method was statistically developed and validated as per ICH guidelines and can be applied for routine quality control analysis of Perindopril and Indapamide in Pharmaceutical dosage forms.


Cite this article:
G. Saravanan, Md. Yunoos, B. Parveena. Development and validation of RP-HPLC method for simultaneous analysis of Perindopril and Indapamide in bulk and combined pharmaceutical dosage form. Asian J. Research Chem. 7(2): February 2014; Page 182-186.


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