Bhavani Ventrapragada, S. Ashutosh Kumar, Manidipa Debnath, N. Sai Krishna, J. Saravanan, V. Greeshma, Ch. Naga Madhusudhan Rao
Bhavani Ventrapragada1*, S. Ashutosh Kumar1, Manidipa Debnath2, N. Sai Krishna3
J. Saravanan3, V. Greeshma3, Ch. Naga Madhusudhan Rao1
1Department of Pharmaceutical Analysis and Quality Assurance, A.K.R.G College of Pharmacy, Nallajerla, West Godavari, 534112, A.P
2Department of Pharmaceutics, A.K.R.G College of Pharmacy, Nallajerla, West Godavari, 534112, A.P
3Department of Pharmacology, A.K.R.G College of Pharmacy, Nallajerla, West Godavari, 534112, A.P
Volume - 7,
Issue - 2,
Year - 2014
Objective: A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of Aceclofenac and Paracetamol in solid dosage form. Method: The method was carried out on a Cosmicsil Adze c18 (150x4.6nm) 5micrometer column with a mobile phase consisting of.2M pH 7 phosphate buffer and acetonitrile in 75: 25 v/v respectively at a flow rate of 1ml/min. Result: Detection was carried out at 265 nm. The retention time of Aceclofenac and Paracetamol was 6.03 and 2.80minutes respectively. The % assays for the drugs Paracetamol and Aceclofenac were found to be 100.6% and 98.5% respectively and specified within the acceptance limits. Conclusion: Hence the developed method can be routinely used for the simultaneous estimation of Paracetamol and Aceclofenac in the marketed formulations. The lowest possible concentrations of Paracetamol and Aceclofenac that can be detected by the present method were found to be 0.652153µg/ml and 0.364186µg/ml respectively and that can be Quantitated were found to be 1.976221µg/ml and 1.103594µg/ml respectively.
Cite this article:
Bhavani Ventrapragada, S. Ashutosh Kumar, Manidipa Debnath, N. Sai Krishna, J. Saravanan, V. Greeshma, Ch. Naga Madhusudhan Rao. A New Simple and Accurate Method Development and Validation for Simultaneous Estimation of Aceclofenac and Paracetamol in Solid Dosage Form by Using RP –HPLC. Asian J. Research Chem. 7(2): February 2014; Page 233-242.