M.L.N. Acharyulu, P.V.S.R. Mohana Rao, P. Jaya Ranga Rao
M.L.N. Acharyulu1*, P.V.S.R. Mohana Rao2, P. Jaya Ranga Rao3
1Associate Professor, Centurion University of Technology and Management, Andhra Pradesh - 530017, India.
2Research Scholar, Department of Engineering Chemistry, A.U. College of Engineering (A), Visakhapatnam.
3Assistant Professor, Department of Chemistry, Basic Sciences and Humanities, Baba Institute of Technology and Science, Visakhapatnam, Andhra Pradesh- 530017, India.
Volume - 13,
Issue - 6,
Year - 2020
Two simple and sensitive extractive visible spectrophotometric methods (A and B) for the assay of Darunavir (DNV)and Methoxamine hydrochloride (MHC), in pure and pharmaceutical formulations based on under acidic conditions the primary amine group in DNV and MHC forming coloured condensation product with aromatic aldehyde Isatin forms the basis for Method-A and Method-B. The coloured products exhibit absorption ?max at 412nm and 450nm for methods A and B respectively. Regression analysis of Beer-Lambert plots showed good correlation in the concentration ranges (100-600)µg/ml for method A, (50-300)µg/ml for method B and correlation co-efficients are 0.994(A), 0.9967(B) respectively. The Sandell’s sensitivities are2.0044x 104, 1.8991 x 104 (1 mole cm-1) and molar absorptivity values are 2.7322x 10-2, 1.3043 x 10-2(µgcm¬-2). The proposed methods are applied to commercial available formulations and the results are statistically compared with those obtained by the UV reference method and validated by recovery studies. The results are found satisfactory and reproducible. These methods are applied successfully for the estimation of theDarunavir and Methoxamine hydrochloride in the presence of other ingredients that are usually present in formulations. These methods offer the advantages of rapidity, simplicity and sensitivity and low cost without the need for expensive instrumentation and reagents.
Cite this article:
M.L.N. Acharyulu, P.V.S.R. Mohana Rao, P. Jaya Ranga Rao. Analytical Applications of Isatin in the Determination of Darunavir and Methoxamine Hydrochloride. Asian J. Research Chem. 2020; 13(6):409-414. doi: 10.5958/0974-4150.2020.00074.7
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