ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Souad Belakehal, Brahim Labed, Louiza Zenkhri, Khedidja Benzahi, Ahmed Tabchouche

Email(s): louizazenkhri@yahoo.fr

DOI: 10.52711/0974-4150.2021.00049   

Address: Souad Belakehal1,3, Brahim Labed2, Louiza Zenkhri3, Khedidja Benzahi2, Ahmed Tabchouche1
1Dynamics, Interaction and Systems Reactivity Laboratory, Process Engineering Department, Faculty of Applied Sciences, University Kasdi Merbah, Ouargla 30000, Algeria.
2Ecole Normale Supérieure Ouargla, Valorisation and Promotion of Saharan Resources laboratory (VPRS), Kasdi Merbah University, Ouargla, Algeria.
3Valorisation and Promotion of Saharan Resources laboratory (VPRS), Chemistry Department, Faculty of Mathematics and Material Sciences, Kasdi Merbah University, Ouargla, Algeria.
3Chemistry Department, Faculty of mathematics and Matter sciences, University Kasdi Merbah.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Ethanolic extracts of Pergularia tomentosa L. were used for the relative analysis of antioxidant and antimicrobial activity against two types of Gram-negative bacteria. (Escherichia coli G (-) and Pseudomonas aeruginosa G (-)) and a gram positive (Staphylococus aureus G (+)). Maximum activity was recorded against Escherichia coli, with a maximum inhibition diameter of 23mm using the disk diffusion method. In addition, the results revealed a higher bacterial activity against Escherichia coli with an inhibition diameter of 23mm compared to standard antibiotics Ampicillin/Nystatin with an inhibition diameter of 11.75mm. The study of the antioxidant activity by the DPPH and FRAP methods showed that vitamin C is the most active with an IC50 equal to 0.010 g/ml, followed by the crude extract, with a value of 0.012 g/ml and finally Tocopherol (VE) which seems to be the least effective with an IC50 of 0.015 g/ml. The results obtained in the present study suggest that Pergularia tomentosa L. can be used in the treatment of diseases caused by the organisms tested. Further chemical and pharmacological investigations can be carried out to isolate and identify the chemical constituents in selected plants responsible for antimicrobial activity.


Cite this article:
Souad Belakehal, Brahim Labed, Louiza Zenkhri, Khedidja Benzahi, Ahmed Tabchouche. In Vitro Comparative Study on the Antibacterial and the Antioxidant Activity of Pergularia tomentosa L. Asian Journal of Research in Chemistry. 2021; 14(4):285-1. doi: 10.52711/0974-4150.2021.00049

Cite(Electronic):
Souad Belakehal, Brahim Labed, Louiza Zenkhri, Khedidja Benzahi, Ahmed Tabchouche. In Vitro Comparative Study on the Antibacterial and the Antioxidant Activity of Pergularia tomentosa L. Asian Journal of Research in Chemistry. 2021; 14(4):285-1. doi: 10.52711/0974-4150.2021.00049   Available on: https://ajrconline.org/AbstractView.aspx?PID=2021-14-4-10


REFERENCES:
1.    Ashakkumar R., Ramaswamy M. J. (2013).  Chem Bio Physci Sec., 3 (2): 1279-1289.
2.    Bindu J., Anjali K., Vibhor K. J. (2011). Asian Journal of Biochemical and Pharmaceutical research. 2(1): 437-452.
3.    Pelt J. M., (2001). Les noveeaux actifs naturels. Marabout, Paris
4.    Babamer Z., Sekhri L., Al-Jaber Hala I., Al-Qudah M. A., Abu Zarga M. H. (2012). Journal of Asian Natural Products Research. 1-7.
5.    Abu Zarga, M. H., Jaber H. I., Babamar Z. Y., Sekhri L., Al-Qudah M. A., Al-humaid Y. G., Abaza I. F., Afifi F. U., (2013). Chemical Composition, Antimicrobial and Antitumor Activities of Essential Oil of Ammodaucus leucotrichus Growing in Algeria.  Journal of Biologically Active Products from Nature, 3 (3): 224-231.
6.    Dia, O. (2019), Optimization de l’extraction des polyphenols des (Phoenix dactylifera) L. par differaents solvents et mèthodes, Ph. D Thesis, University of Ouargla, Algeria.
7.    Dia O., Ouahrani M. R., Laouini S. L., (2018), J Fundaman Appl Sci., 10 (3): 376-393.
8.    Phytochemical Screening, Anti-Bacterial and Antioxidant Activities of some Aerial parts extracts in Atriplex Halimus L., from Ouargla (Algeria)Samira Gattouche, Louiza Zenkhri, Mohammed Lakhdar Befar, Ahmed Tabchouche., Asian J. Research Chem. 13(5):
9.    Mohamed Lakhdar Belfar, Touhami Lanez, Abdekarim Rebiai, Zineb Ghiaba, Evaluation of antioxidant capacity of propolis collected in various areas of Algeria using electrochemical techniques, nt. J. Electrochem. Sci Volume 10 Issue 11 Pages 9641-9651
10.    Ishrak K., Ahmed D. The effecincy of random versus ethno-directed research in the evaluation of sinai medicinal plants for bioactive   compounds. (2000). J. of Ethnopharmacology, 71: 365-376.
11.    Benkeblia N., (2004). Antimicrobial activity of essential oil extracts of various onions (allium cepa) and garlic (Allium stivum). Lebensm-Wiss u-Technol. 37: 263-275.
12.    Imen Lahmar, Hafedh Belghith, Ferjani Ben Abdallah, and Karima Belghith, Utritional Composition and Phytochemical, Antioxidative, and Antifungal Activities of Pergularia tomentosa L., Biomed Res Int. 2017
13.    Haddioui A., Baaziz M., (2001) Population of Atriplex halimus of Moroco Euphyca, Atriplex halimus L. 121: 99-106
14.    Rayyan W. A, Alshammari S. A. G, AL-Sammary A. M. F, AL-Shammari M. S. S, Seder N, Qatoosh L. F. A, Bostami M, Mansoor K, Hamad M. F, Al-Majali I. S, Daiyyah W. A. The Phytochemical Analysis and Antimicrobial Activity of Pergularia Tomentosa in North East Kingdom of Saudi Arabia KSA. Biomed Pharmacol J 2018; 11(4).
15.    A guide to medicineal plants in North Africa, (2005). International Union for Conservation of Nature and Natural ReSources (IUCN).
16.    Biola F., Alogninouwa T., El Bahri L., Ali M., Kaboret Y., Fayoumi B., (1993), Revue d’Elevage et de Medecine Veterinaire des Pays Tropicaux 46, 591-598.
17.    Doaigey A., (1991). Am. J. Bo. 78: 1608-1614.
18.    Shabana M., Mirhom Y., Genenah A., E. Aboutabl E., Amer H., (1990). Aechiv fur Exprimentelle Veterinarmedizin, 44: 389-397.
19.    Al-Farraj A. S., Al-Wabel M. I. (2007).  J. App. Sci. 7: 1170-1181
20.    Al Quran S., (2005). Toxicon. 46: 119-130
21.    Husseine H. I., Alrajhy D., Hashem S. M., (1999). Int. J. Pest. Manag. 45: 211-220.
22.    Nabil A. M. and Anwar M., (2017). phytochemical and pharmacological activities of Pergularia tomentosa L. - A review. AJPS,4(11), 4558-4565
23.    Hammiche H. I., Maiza K., (2006). J. Ethnophamacol. 105: 358-366.
24.    Arafa I., Alberto P., Balestrieri M., Mahalel A., Springuel I., Oleszek W., Pizza C., Piacente S., (2006).  J. Nat. Prod. 69: 1319-1327.
25.    Piacente S., Masullo M., De Neve N., Dewelle J., Hamed A., Kiss R., (2009). J. Nat. Prod. 72: 1087-1095.
26.    Babaamar Z., Sekhri L., AlJaber H., Al-Qudah M. A., Abu Zarga M. H., (2013). Annales des Sciences et Technologie, 5: 1-5.
27.    Harbone J. (1973). Phytochemical methods. Chapman and Hall. London
28.    Swarnamoni D., Mukundam B., Shagufa A., (2013). Asian Pharm Clin Res., 6 (4): 136-139.
29.    Harbone J. B., (1984). Phytochemical methods. A guide to modern techniques of plant analysis. 2nd ed., Chapman and Hall. London. P: 288.
30.    Bassole I. H.  N., Gnoula C., Roger N., Yonli A., Morel L., Figueredo J., Jean-Marc A. L., Simpore J., (2014) Chemical Composition, Antioxidant, Anti-Inflammatory and Anti-Proliferative Activities of Essential Oils of Plants from Burkina Faso Bagora Bayala 9: e92122.
31.    Uchiyama K., Suzuki Y., (1968) Fukuzawa Etude Biochimiaues. 88: 68 – 683.
32.    Benzie I. F. F., Stain N. J. J., (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, FRAP essay, Anal   Biochem., 239: 70-76.
33.    Baurea A. W., Kirry W., M. M., Sherres J. C. A., Turch M. (1966). Antibiotic susceptibility testing by a standardized single disk method, Amer J. Clin Pathol., 45: 493 – 496.     
34.    Allaoui A., Cherity A., Al-Gharabli S., Gherraf N., Chebouat E., Dadamoussa, B. A., Al-Lahhm A. (2014). Research Journal of pharmaceutical and Biological and Chemical Sciences. 5 (5): 85-89.
35.    Al-Abed K. F., (2008). Antibacterial activity in the volatile oils of some medicinal plants in Saudi Arabia. Microbiology, aureus, Bacillus, Escherichia coli, outside the body of the organism. M. Sc Thesis, Faculty of Education, University of Riyadh; Faculty of Education, University of Riyadh.
36.    Nanasombat S., Wimuttigsol P. (2011) Food Sci, Biotechnol., 20: 45-53.
37.    Jukie M., Milos M., (2005). Croatica Acta, 78: 105-110.

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