ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Syed Sara Afreen, Durga Panikumar Anumolu, Pulusu Veera Shakar, Ceema Mathew, Devilal Jarpula, Rahul Nalluri

Email(s): panindrapharma05@gmail.com

DOI: 10.52711/0974-4150.2023.00048   

Address: Syed Sara Afreen1, Durga Panikumar Anumolu1*, Pulusu Veera Shakar2, Ceema Mathew1, Devilal Jarpula3, Rahul Nalluri4
1Gokaraju Rangaraju College of Pharmacy, Department of Pharmaceutical Analysis, Osmania University, Hyderabad, Telangana-500090, India.
2Ohio University, Department of Chemistry and Bio chemistry, Athens, OH, USA-45701
. 3Gokaraju Rangaraju College of Pharmacy, Department of Pharmaceutical Chemistry, Osmania University, Hyderabad, Telangana-500090, India.
4Independent Researcher, Osmania University, Hyderabad, Telangana-500090, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 4,     Year - 2023


ABSTRACT:
Two simple, specific, accurate, precise, sensitive and cost-effective spectrophotometric methods have been developed and validated for quantification of alogliptin in pure form and pharmaceutical formulations. Method 1 is based on the measurement of absorbance of bluish green coloured chromogenic complex at 452 nm which is formed by reaction of alogliptin with ferric chloride and potassium ferricyanide (redox technique). Method-2 is based on the oxidative coupling reaction of MBTH with alogliptin in the presence of ferric chloride to form green coloured chromogen with absorption maximum at 608 nm. The methods obeyed Beer’s law in concentration range of 5-30 µg/ml and 20-100 µg/ml with good correlation coefficients of 0.9994 and 0.999 for method 1 and 2 respectively. The limit of detection, limit of quantification and sandell’s sensitivity values are reported for all the two spectrophotometric methods. The developed method was validated statistically as per ICH guidelines. No significant interference was observed from the excipients commonly used as pharmaceutical aids with the assay procedure. The reliability of both the methods is further ascertained by performing recovery tests by standard addition method. The developed method is simple, sensitive, specific and can be successfully employed in routine analysis of alogliptin in pharmaceutical dosage forms.


Cite this article:
Syed Sara Afreen, Durga Panikumar Anumolu, Pulusu Veera Shakar, Ceema Mathew, Devilal Jarpula, Rahul Nalluri. Chromotag application in quantification of alogliptin. Asian Journal of Research in Chemistry 2023; 16(4):291-5.. doi: 10.52711/0974-4150.2023.00048

Cite(Electronic):
Syed Sara Afreen, Durga Panikumar Anumolu, Pulusu Veera Shakar, Ceema Mathew, Devilal Jarpula, Rahul Nalluri. Chromotag application in quantification of alogliptin. Asian Journal of Research in Chemistry 2023; 16(4):291-5.. doi: 10.52711/0974-4150.2023.00048   Available on: https://ajrconline.org/AbstractView.aspx?PID=2023-16-4-7


REFERENCES:
1.    M.N. Piero1, G.M. Nzaro. Diabetes mellitus – a devastating metabolic disorder. Asian Journal of Biomedical and Pharmaceutical Sciences. 2014, 4(40), 1-7.
2.    P.D. Anumolu, G. Sunitha, B. Priyanka, G. Mounika, A. Shilpa. Analytical QbD approach first order model- robust RP-HPLC method for simultaneous assessment of alogliptin and metformin. Indian Drugs, 2021, 58 (05), 58-67.
3.    V.D. Gaitonde. A rapid and sensitive validated reverse phase high performance liquid chromatography method for determination of genotoxic impurity 2-(bromomethyl) benzonitrile in alogliptin benzoate. International Journal of Innovative Pharmaceutical Sciences and Research. 2016, 4(7),803-816
4.    B.P. Patel, R.C. Mashru. Sensitive and selective approaches for real time estimation of alogliptin benzoate and metformin hydrochloride in synthetic mixture. Int Bull Drug Res. 2014,4(6),148-159.
5.    R. Anjali, P. Paresh. Spectrophotometric estimation of Glibenclamide and Alogliptin in synthetic mixture by area under curve method. Int. J. Pharm. Pharmacist. Res. 2016,6,228-236.
6.    U.Raval Kashyap, Srinivasa, Shweta Dadhaniya. First order derivative and dual wavelength spectrophotometry methods development and validation for simultaneous estimation of alogliptin and pioglitazone in bulk and dosage form. Journal of Pharmaceutical Chemistry. 2014, 2(4),1-20.
7.    P.J. Yadav, V.N. Kadam, S. K. Mohite. Development and Validation of UV spectrophotometric method for alogliptin Benzoate in Bulk Drug and Tablet Formulation. Journal of Current Pharma Research, 2014,4 (4),1286-1290.
8.    P. Bhumik, C. Rajashree, mashru. Sensitive and selective approaches for real time estimation of alogliptin benzoate and metformin hydrochloride in synthetic mixture. International Bulletin of Drug Research.2014, 4(6),148-159.
9.    A.D. Pani Kumar, A. Kavitha, D. Vijaya Durga, S. Himabindu , G. Sunitha, K. Ramakrishna. Spectrophotometric quantification of tadalafil by oxidative coupling reaction with MBTH reagent. Anal Chem Ind J. 2013,13(9),361-364.
10.    A. Shravya, R.S. Chandan, B.M. Gurupadayya, M. Sireesha, S. Adeunutula, Spectrophotometric determination of ezetimibe using MBTH reagent in pharmaceutical dosage form. Int. J. Res. Ayurveda Pharm. 2011, 2(2),521-525.
11.    A.D. Pani Kumar, G. Archana, G. Sunitha, K. Rachel Paul, R. Harika, N.S. Sowndarya. Simplistic application of 3-methy-2-benzothiazoline hydrazone (MBTH), an oxidative coupling chromogenic reagent for quantification of metaxalone and dabigatran etexilate mesylate bulk drug and their dosage forms. Pharm Anal Acta. 2015, 6(5).
12.    International Conference on Harmonization, Harmonized Tripartite Guideline, Q2(R1) (2005). Validation of Analytical Procedures, Text and Methodology

Recomonded Articles:

Author(s): Ravi N. Patel, Urviben Y. Patel, Kiran M. Patel, Jimit S. Patel, Ankit D. Patel, Dhrubo Jyoti Sen

DOI:         Access: Open Access Read More

Author(s): Chavan Pooja Ajit, Avinash Mahadeo Bhagwat, Atul Prabhakar Chaudhari

DOI: 10.52711/0974-4150.2021.00061         Access: Open Access Read More

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Nirav C. Patel, Dipti B. Patel, Pruthviraj K. Chaudhari

DOI:         Access: Open Access Read More

Author(s): Nachiket S. Dighe, Priyanka R. Varade, Ganesh S. Shinde, Priya S. Rao

DOI: 10.5958/0974-4150.2019.00028.2         Access: Open Access Read More

Author(s): PA Jadhav, CS Raut, JP Bidada, BB Buwa, PN Dhabale, SC Dhawale

DOI:         Access: Open Access Read More

Author(s): Sushil D. Patil, Tejashri Dugaje, Sanjay J. Kshirsagar

DOI: 10.5958/0974-4150.2019.00024.5         Access: Open Access Read More

Author(s): Alka Verma, Bhupesh Verma, Sunil Kumar Prajapati, Kishu Tripathi

DOI:         Access: Open Access Read More

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags