Author(s):
Nazira Sarkis, Ghalia Sabbagh, Hazar Abdallah
Email(s):
nazirasarkis@gmail.com , ghaliaaa@hotmail.com , hazarabdallah49@gmail.com
DOI:
10.52711/0974-4150.2025.00046 
Address:
Nazira Sarkis1*, Ghalia Sabbagh2, Hazar Abdallah1*
1Department of Analytical and Food Chemistry, Faculty of Pharmacy, Aleppo University, Syria.
2Department of Pharmaceutical Chemistry and Quality Control, Faculty of Pharmacy, Aleppo University, Syria.
*Corresponding Author
Published In:
Volume - 18,
Issue - 5,
Year - 2025
ABSTRACT:
Many previous studies have shown that excessive consumption of food products containing sodium benzoate may cause many side effects that can be harmful to health. Thus, it is critical to evaluate the amount of benzoic acid and its salts in food products by inexpensive and uncomplicated method for repeated examination. So, in this study, we determined the optimal conditions for extracting benzoates from food products available in local markets in order to determine their proportions and ensure that they are within the permissible limits. Various conditions were studied (solvent, acid, acid concentration and volume, temperature and volume of the solvent used). The best results were obtained by using hexane as a solvent in a volume of 30ml in presence of phosphoric acid at a concentration of (0.70M) at laboratory temperature. The analytical method has been validated and R² was (0.9995), The standard deviation ranged between (0.94-1.85)% over one day, and ranged between (0.55-1.10)% over three consecutive days. The percentage of benzoates was also measured in a commercial some sample beverages; it was found that the amount of benzoate present in it was within the acceptable limits according to the standard specification.
Cite this article:
Nazira Sarkis, Ghalia Sabbagh, Hazar Abdallah. Simple Spectrophotometric Method for Determination of Sodium Benzoate in some Beverages. Asian Journal of Research in Chemistry. 2025; 18(5):303-0. doi: 10.52711/0974-4150.2025.00046
Cite(Electronic):
Nazira Sarkis, Ghalia Sabbagh, Hazar Abdallah. Simple Spectrophotometric Method for Determination of Sodium Benzoate in some Beverages. Asian Journal of Research in Chemistry. 2025; 18(5):303-0. doi: 10.52711/0974-4150.2025.00046 Available on: https://ajrconline.org/AbstractView.aspx?PID=2025-18-5-1
7. REFERENCES:
1. Galal m. Elmanfe. Spectrophotometric Determination of Sodium Benzoate in Some Soft Drinks Collected from Some Local Markets in El-bieda City – Libya. International Journal of Applied Science. 2020; 6(2).
2. Ukwo SP, Udo II, Ndaeyo N. Food additives: overview of related safety concerns. Food Sci Nutr Res. 2022; 5(1): 1. https://doi.org/10.33425/2641-4295.1052
3. Carocho M, Barreiro MF, Morales P, Ferreira IC. Adding molecules to food, pros and cons: A review on synthetic and natural food additives. Comprehensive Reviews in Food Science and Food Safety. 2014; 13(4): 377–99. https://doi.org/10.1111/1541-4337.12065.
4. Patanwala. F, Hariramani. K and Valecha .SH, Analysis of specific parameters of selected food products, Asian Journal of Research in Chemistry. 2016; 9(8).
5. Gould, G. W.; “Preservation: past, present and future”. British Medical Bulletin. 2000, 56(1), 84-96.
6. Antakli S, Alahmad A and Badingki H. Simultaneous determination of sodium benzoate and potassium sorbate preservatives in foodstuffs using High performance liquid chromatography, Asian Journal of Research in Chemistry. 2010; 3(1).
7. Erkmen O, Bozoglu TF. Section I: Food preservation techniques. Chapter 5: Food preservation by additives and biocontrol. In: Erkmen O, Bozoglu TF, editors. Food microbiology: principles into practice. Hoboken: John Wiley & Sons, Inc.; 2016. [Google Scholar]
8. Gibson CM. Benzoic Acid. WikiDoc. 2012.
9. Neumuller OA. Rompps Chemie-Lexikon. 6th ed. Stuttgart: Frankh’sche Verlagshandlung; 1988.
10. Penney V, Henderson G, Blum C, et al. The potential of phytopreservatives and nisin to control microbial spoilage of minimally processed fruit yogurts. Innov Food Sci Emerg Technol. 2004; 5: 369–375.
11. GSFA Online Food Additive Group. Details: Benzoates; 2006
12. European Parliament and Council Directive No 95.2/EC of 20 February 1995 on Food Additives Other than Colours and Sweeteners; 1995.
13. Linke BG, Casagrande TA, Cardoso LA. Food additives and their health effects: A review on preservative sodium benzoate. Afr J Biotech. 2018; 17(10): 306–10. https://doi.org/10.5897/AJB2017.16321.
14. Pravalika. M and Archana. J. A new validated spectroscopic method for the estimation of sodium benzoate in soft drinks. Asian Journal of Pharmaceutical Analysis. 2022; 12(1).
15. Lakshmi Narayanan Venu and Anoop Austin. Study and Quantification of Preservatives [E211] In Carbonated Soft Drinks Samples. Journal of Applied Chemistry. 2019; 12(4): 17-23.
16. Gören A.C, Bilsel G, Simsek A, Bilsel M, Akçadag F, Topal K, Ozgen H. HPLC and LC–MS/MS methods for determination of sodium benzoate and potassium sorbate in food and beverages: Performances of local accredited laboratories via proficiency tests in Turkey. Food Chemistry. 2015; 175: 273–279
17. Mirshekari S, Moeini S, Bahri A, Safari R, Saeidi Asl M. Effect of Bacteri-ocine Z And Sodium Benzoate on Shelf-Life of Caspian Roach (Rutilus Frisii Kutum) Fillet. J Food Sci Technol. 2010; 2(1): 19–27.
18. Boukarim C, Jaoudé SA, Bahnam R, et al. Preservatives in liquid pharmaceutical preparations. Drug Test Anal. 2009; 1: 146–148.
19. Ala Yahya Sirhan. Optimization and validation of an HPLC – UV method for determination of benzoic acid and sorbic acid in yogurt and dried –yogurt products using a design of experiment. Applied Science Private University. 2018; 18(3): 522-530.
20. Kalva S. and Ganesh S. Determination and estimation of preservatives in packaged food, Asian Journal of Pharmaceutical and Clinical Research. 2024; 17(1).
21. Afshar M, Moallem SA, Houshang Mohammadpour A, Shiravi A, Majid Jalalian S, Jafar M, Golalipour. Teratogenic effects of carbamazepine on embryonic eye development in pregnant mice. Cutan Ocul Toxicol. 2010; 29: 10–5. 10.3109/15569520903380353.
22. Walczak-Nowicka LJ. M. Herbet. Sodium Benzoate-Harmfulness and potential use in therapies for disorders related to the nervous system: A review. Nutrients. 2022; 14: 10.3390/nu14071497.
23. Piper JD, Piper PW. Benzoate and sorbate salts: A systematic review of the potential hazards of these invaluable preservatives and the expanding spectrum of clinical uses for sodium benzoate. Compr Rev Food Sci Food Saf. 2017; 16: 868–80. 10.1111/1541-4337.12284.
24. Akter S., Haque khan M, Abu Z and Bari L. Identification and quantification of sodium benzoate in different brands of mango juices available in tangail region. Bangladesh Pharmaceutical Journal. 2017; 20(1): 20-26.
25. Alabyada M. HPLC method for the determination of benzoic acid in samples of commercial children’s food in the markets of aden-Yemen, Journal of Science and Technology. 2023; 28(2).
26. Shaikh S., Kalshetti M. and Patil R. HPLC method development for simultaneous estimation of sodium benzoate and potassium sorbate in food products, Asian Journal of Applied Science and Technology. 2018; 2(2).
27. Vyas. A et al. Recent applications of UV-visible derivative spectroscopic method. Asian Journal of Pharmaceutical Analysis. 2023; 13(2).
28. Pravalika. M and Archana. J. Simultaneous estimation of sodium benzoate and caffeine in soft drinks by UV spectroscopy. Research Journal of Pharmacy and Technology. 2023; 16(5).
29. Bhange. P, Baokar. SH and Patil. R. Quantifying caffeine content in green tea and soft drink using UV- visible spectrophotometry. Asian Journal of Pharmaceutical Analysis. 2024; 14(3).
30. Kathirvel. S et al. An overview of sample preparation techniques for food analysis. Asian Journal of Pharmaceutical Analysis. 2017; 7.
31. Singh. G, Vuruputor. V and Kalaichelvan. C. A nutrition dense additive for processed food items. Research journal of pharmacy and technology. 2018; 11(6).
32. Bendre. S, Shinde. K, Kale. N and Glida. S, Artificial intelligence in food industry: a current panorama, Asian journal of pharmacy and technology, 2022, 12(3).
33. Slavkova. M et al. Effect of soft beverages on the in –vitro dissolution of gastro resistance Tablets containing low dose acetylsalicylic acid. Research Journal of Pharmacy and Technology. 2021; 14(12).