A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH2PO4 is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05M KH2PO4 + 0.1% H3PO4 adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250? 4.6mm) and 5? particle size with rheodyne injector. The flow rate was 1ml/min and uv detection at 238nm. The retention time for atenolol was 4min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.
Cite this article:
Amrish Sharma, Mukul Tailang, Sonali Khare, Tripti Shukla. RP-HPLC Determination of Atenolol in Its Pharmaceutical Dosage Forms. Asian J. Research Chem. 2(4):Oct.-Dec. 2009 page 485-487.