A simple spectrophotometric method has been developed for the determination of Ni (II) by using Isatin-3- Semicarbazone (HISC) as an analytical reagent. HISC has been synthesized and characterized by elemental and spectral analysis. HISC extracts Ni (II) quantitatively (99.80%) into iso - amyl alcohol from an aqueous solution of pH range 5.5 - 7.2. Iso - amyl alcohol extract shows an intense peak at 500 nm (? max). Beer’s law is obeyed over the Ni (II) concentration range of 1.0 - 6.0 µg/ml. The sandell’s sensitivity and molar absorptivity for Ni - HISC system is 7.32 ngcm-2 and 8014.07 L mole-1cm-1 respectively. The composition of extracted species is found to be 1: 2 (Ni: HISC) by Job’s Continuous Variation and Mole Ratio Method. Interference by various ions has been studied. The proposed method has been successfully applied for determination of Ni (II) in alloy samples.
Cite this article:
Prashant A. Borade, Anandkumar S. Gupta, V. D. Barhate. Development of Extractive Spectrophotometric Determination of Nickel (II) with Isatin-3-Semicarbazone (HISC) as an Analytical Reagent. Asian J. Research Chem. 4(12): Dec., 2011; Page 1905-1907.