ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Ravindra Pandey, Swarnlata Saraf, S. Saraf

Email(s): ravindra56@rediffmail.com

DOI: Not Available

Address: Ravindra Pandey*, Swarnlata Saraf, S. Saraf
University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010 – India
*Corresponding Author

Published In:   Volume - 4,      Issue - 12,     Year - 2011


ABSTRACT:
A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method developed and validated for the determination of Cinnamaldehyde in Indian traditional formulation (sitopaladi churna) and crude drug extracts. Analysis was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of Toluene: Ethyl acetate: Methanol (8:1:1) at room temperature (25±2 ?C). Camag TLC scanner III was used for spectrdensitometric scanning and analysis in absorbance mode at 295 nm. The system was found to give compact spots for Cinnamaldehyde (Rf value of 0.55±0.02). The linear regression analysis data for the calibration plots showed good linear relationship (r2 = 0.996 ±0.0003) in the concentration range 200–1200 ng spot-1 with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines, the method was validated for precision, recovery, robustness and ruggedness. The limits of detection and quantification were determined. The Cinnamaldehde content was quantified and estimated from the formulation and the cinnamomum zeylanicum plant part. Statistical analysis of the data showed that the method is reproducible and selective for the quantitatve determination of Cinnamaldehyde.


Cite this article:
Ravindra Pandey, Swarnlata Saraf, S. Saraf. TLC Densitometry Method for Determination of Cinnamaldehyde in a Traditional Indian Formulation. Asian J. Research Chem. 4(12): Dec., 2011; Page 1953-1956.

Cite(Electronic):
Ravindra Pandey, Swarnlata Saraf, S. Saraf. TLC Densitometry Method for Determination of Cinnamaldehyde in a Traditional Indian Formulation. Asian J. Research Chem. 4(12): Dec., 2011; Page 1953-1956.   Available on: https://ajrconline.org/AbstractView.aspx?PID=2011-4-12-31


Recomonded Articles:

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Prasanna Reddy Battu, MS Reddy

DOI:         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Muralidhar Reddy Avuthu, B. Venkata Reddy, SNVS Murthy

DOI: 10.5958/0974-4150.2016.00076.6         Access: Open Access Read More

Author(s): Sravani A

DOI: 10.5958/0974-4150.2018.00144.X         Access: Open Access Read More

Author(s): Nachiket S. Dighe, Priyanka R. Varade, Ganesh S. Shinde, Priya S. Rao

DOI: 10.5958/0974-4150.2019.00028.2         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Rachana Rani, Sudeep Mishra

DOI:         Access: Open Access Read More

Author(s): Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V

DOI: 10.5958/0974-4150.2019.00062.2         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj S. Charde, Rita M. Charde

DOI:         Access: Open Access Read More

Author(s): Rajesh Balkrishna Tawade, Abha Sham Dhomane

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags