Ujjaval N. Limbachiya, Nikita N. Patel, Rana Nikesh, Rajesh K.S, Shreya Patel, Gajanan Shinde.
Ujjaval N. Limbachiya*, Nikita N. Patel, Rana Nikesh, Dr. Rajesh K.S, Shreya Patel, Gajanan Shinde.
Parul Institute of Pharmacy, Waghodia, Limda, Gujarat, India
Volume - 6,
Issue - 4,
Year - 2013
An Absoption Correction UV spectrophotometric and a reversed Phase high performance liquid chromatographic method for the determination of Ambroxol and Olopatadine Hydrochloride in Synthetic mixture were developed. The Absorption Correction UV spectrophotometric method was found to be accurate with 99.75±0.04 recovery for ambroxol and recovery for olopatadine 99.89±0.02 respectively and method is also precise with a coefficient of variation for olopatadine and ambroxol was 0.996 and 0.885 respectively. For reverse phase HPLC, A reversed phase C18 column with methanol: ammonium acetate buffer (80:20, v/v, pH 5.5) mobile phase was used and the detector wavelength was set at 244 nm. Linearity range for ambroxol and Olopatadine Hcl was found to be 78-150 ?g/ml and 13-25 ?g/ml respectively.
Cite this article:
Ujjaval N. Limbachiya, Nikita N. Patel, Rana Nikesh, Rajesh K.S, Shreya Patel, Gajanan Shinde.. Analytical Method Development and Validation for Simultaneous Determination of Ambroxol and Olopatadine Hydrochloride in Synthetic Mixture. Asian J. Research Chem. 6(4): April 2013; Page 389-392.