ABSTRACT:
In this work the solid phase microwave assisted synthesis of bis-hetrocyclic chalcones were carried out by reaction of previously synthesized (4-methylphenyl) pyrrolidine-2,5-dione 1 and (4-Chlorophenyl) pyrrolidine-2,5-dione 4 with different substituted benzaldehydes 2a-e in presence of neutral alumina upon microwave irradiation afforded to bis-heterocyclic chalcones 3a-e and 5a-e. This chalcone further treatment with semicarbazide hydrochloride in presence of neutral alumina solvent free condition upon microwave irradiation leading to ring closure and furnished in to dipyrazole dicorboxamide derivative 6a-e and 7a-e. The structures of all synthesized compounds were determined by using FT- IR and 1HNMR spectroscopy technique. The anti microbial activities of all dipyrazole dicorboxamide derivatives were tested against selective pathogenic microorganisms of gram positive and gram negative bacterial strains similarly antifungal evaluation were determined against pathogenic fungal strains by using disc diffusion method. The zone of inhibition shown by synthesized dicorboxamide derivative were measured in mm and compared with standard antibiotic drug Chloramphenicol and Amphotericine-B. Some compounds dose not shows antibacterial activities some of them are microbially active and the compound 7-(4-chlorophenyl)-3,4-bis(4-methoxyphenyl)-3,3a,3b,4-tetrahydro-2H-pyrrolo[2,3-c:5,4-c']dipyrazole-2,5(7H)-dicarboxamide 7d exhibited good antifungal activity towards fungal strain Candida Albicans and similarly it display promising antifungal activity against pathogenic fungal species Aspergillus Niger at minimum inhibitory concentration of 100 µgm per disc.