Author(s):
Fathi Guemari, Salah Eddine Laouini, Abdelkrim Rebiai, Abderrhmane Bouafia
Email(s):
abdelrahmanebouafia@gmail.com
DOI:
10.5958/0974-4150.2020.00037.1 
Address:
Fathi Guemari, Salah Eddine Laouini, Abdelkrim Rebiai, Abderrhmane Bouafia
University of Echahid Hamma Lakhdar, El Oued 39000, Algeria.
*Corresponding Author
Published In:
Volume - 13,
Issue - 3,
Year - 2020
ABSTRACT:
In this study, we were devoted to study this plant Silybum marianum (L.) we divided it into four parts of leaves, twigs, flowers, and grains. Among the criteria that led to the selection of this plant, is the geographical origin and traditional use of this plant, which affects the natural composition of the plant, our research focuses on the identification of phenolic compounds (polyphenols, flavonoids, and flavanol), evaluation of antioxidant and activity antimicrobial For the four samples of plant Silybum marianum (L.) .The determination of the total polyphenols (TPC) by the method of Singleton and Ross using the Folin-Ciocalteu reagent, total flavonoid (TFC) by the method aluminum chloride method Ordonnez, and The estimation of the total flavanol content in the various extracts is carried out contained is carried out by the method of Mbaebie et al, The total antioxidant capacity (CAT) of the extracts is evaluated by the phosphomolybdenum method, DPPH test We use (2,2-diphenyl-1-picrylhydrazyl), The determination of antioxidant capacity by the FRAP method is carried out as follows The FRAP method is basedon the present ferric iron (Fe3+) reduction reaction in the K3Fe (CN)6 ferrous iron (Fe2+) complex by an antioxidant, The results of the separation by high performance liquid chromatography HPLC. Phenol content and flavonoid content and flavanol content of the four plant samples varied and phenol content showed More effective for antioxidant activity and flavonoid content showed More effective for antimicrobial.
Cite this article:
Fathi Guemari, Salah Eddine Laouini, Abdelkrim Rebiai, Abderrhmane Bouafia. Phytochemical screening and Identification of Polyphenols, Evaluation of Antioxidant activity and study of Biological properties of extract Silybum marianum (L.). Asian J. Research Chem. 2020; 13(3):190-197. doi: 10.5958/0974-4150.2020.00037.1
Cite(Electronic):
Fathi Guemari, Salah Eddine Laouini, Abdelkrim Rebiai, Abderrhmane Bouafia. Phytochemical screening and Identification of Polyphenols, Evaluation of Antioxidant activity and study of Biological properties of extract Silybum marianum (L.). Asian J. Research Chem. 2020; 13(3):190-197. doi: 10.5958/0974-4150.2020.00037.1 Available on: https://ajrconline.org/AbstractView.aspx?PID=2020-13-3-7
REFERENCES :
1. Belouahem-Abed D, Belouahem F, Bélair G. Biodiversité floristique et vulnérabilité des aulnaies glutineuses de la Numidie algérienne (NE Algérien). European Journal of Scientific Research. 2009 ;32(3) :329-61.
2. Gairola S, Shariff NM, Bhatt A. Influence of climate change on production of secondary chemicals in high altitude medicinal plants : issues needs immediate attention. Journal of Medicinal Plants Research. 2010 ;4(18):1825-9.
3. Popovici C, Saykova I, Tylkowski B. Evaluation de l’activité antioxydant des composés phénoliques par la réactivité avec le radical libre DPPH. 2010.
4. Polyak SJ, Morishima C, Lohmann V, Pal S, Lee DY, Liu Y, et al. Identification of hepatoprotective flavonolignans from silymarin. Proceedings of the national academy of sciences. 2010 ;107(13):5995-9.
5. Scambia G, De Vincenzo R, Ranelletti P, Panici PB, Ferrandina G, D'Agostino G, et al. Antiproliferative effect of silybin on gynaecological malignancies : synergism with cisplatin and doxorubicin. European Journal of Cancer. 1996 ;32(5) :877-82.
6. Jakkula M, Boucher TA, Beyendorff U, Conn SM, Johnson JE, Nolan CJ, et al. A randomized trial of Chinese herbal medicines for the treatment of symptomatic hepatitis C. Archives of internal medicine. 2004 ;164(12) :1341-6.
7. Saller R, Meier R, Brignoli R. The use of silymarin in the treatment of liver diseases. Drugs. 2001 ;61(14):2035-63.
8. Gangwar K, Deepali GR, Gangwar R. Ethnomedicinal plant diversity in Kumaun himalaya of Uttarakhand, India. Nature and Science. 2010 ;8(5) :66-78.
9. Bouafia A, Laouini SE, Ouahrani MR. A Review on Green Synthesis of CuO Nanoparticles using Plant Extract and Evaluation of Antimicrobial Activity. Asian Journal of Research in Chemistry. 2020 ;13(1) :65-70.
10. Bouafia A, Laouini SE. Green synthesis of iron oxide nanoparticles by aqueous leaves extract of Mentha Pulegium L.: Effect of ferric chloride concentration on the type of product. Materials Letters. 2020 ;265 :127364.
11. Chavan Y, Singhal RS. Ultrasound-assisted extraction (UAE) of bioactives from arecanut (Areca catechu L.) and optimization study using response surface methodology. Innovative food science & emerging technologies. 2013 ;17 :106-13.
12. Waterhouse A. Folin-Ciocalteau micro method for total phenol in wine. American Journal of Enology and viticulture. 1999 ;28 :1-3.
13. Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food and Chemical Toxicology. 2013 ;55:290-6.
14. Lagnika L. Etude phytochimique et activité biologique de substances naturelles isolées de plantes béninoises: Strasbourg 1; 2005.
15. Mbaebie B, Edeoga H, Afolayan A. Phytochemical analysis and antioxidants activities of aqueous stem bark extract of Schotia latifolia Jacq. Asian Pacific Journal of Tropical Biomedicine. 2012;2(2):118-24.
16. Anita A, Retna A, Joseph J. Phytochemical screening and column chromatography studies of Aerva lanata. Asian Journal of Research in Chemistry. 2018;11(1):84-90.
17. Benhammou N. Activité antioxydante des extraits des composés phénoliques de dix plantes médicinales de l’Ouest et du Sud-Ouest Algérien 2012.
18. Shahwar D, Raza MA. Antioxidant potential of phenolic extracts of Mimusops elengi. Asian Pacific journal of tropical biomedicine. 2012;2(7):547-50.
19. Brand-Williams W, Cuvelier M-E, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology. 1995;28(1):25-30.
20. Sánchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food science and technology international. 2002;8(3):121-37.
21. Gülçin İ, Mshvildadze V, Gepdiremen A, Elias R. Antioxidant activity of saponins isolated from ivy: α-hederin, hederasaponin-C, hederacolchiside-E and hederacolchiside-F. Planta medica. 2004;70(06):561-3.
22. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004 ;26(2):211-9.
23. Roginsky V, Lissi EA. Review of methods to determine chain-breaking antioxidant activity in food. Food chemistry. 2005 ;92(2):235-54.
24. Raj AJ, Dorairaj S. Phytochemical Screening and In-vitro Antioxidant Activity of Cissus quadrangualris. Asian Journal of Research in Chemistry. 2010 ;3(4) :876-8.
25. Boutlelis DA, Bordjiba O. Etude phytochimique et activité antimicrobienne, antioxydante, antihépatotoxique du Marrube blanc ouMarrubium vulgare L. [ressource textuelle, sauf manuscrits]. 2014.
26. Rivero-Pérez M, Muniz P, González-Sanjosé M. Contribution of anthocyanin fraction to the antioxidant properties of wine. Food and Chemical Toxicology. 2008 ;46(8):2815-22.
27. Panaiva L, editor Techniques chromatographiques orientées sur les materiaux composites. Conférence Eurocopter Marseille, France; 2006.
28. Ushasri S, Nagaraju K, Prasanthi D, Ramadevi K, Bhargavi C. In Vitro Antihelmintic Activity and Preliminary Phytochemical Screening of Whole Plant of Vernonia cinerea. Asian Journal of Research in Chemistry. 2013;6(1):68-70.
29. Nikhat F, Satynarayanaa D, Joshia AB. Phytochemical and pharmacological investigation of roots of Syzygium cuminii (L) skeel. Asian Journal of Research in Chemistry. 2008;1(1):22-5.
30. Rub RA, Mukadam A, Pinjari J, Nathani A, Sagri A. Pharmacognostic and Phytochemical Evaluation of Quirivelia frutescens (Apocynaceae). Asian Journal of Research in Chemistry. 2009 ;2(4):509-12.
31. Nagarajan M, Christina A, Devi P, Meera R. HPTLC analysis and Phytochemical Investigation of Leaves of Euphoria longan. Asian Journal of Research in Chemistry. 2010 ;3(1):31-5.
32. Majaz Q, Nazim S, Siraj S, Zameeruddin M, Khan R. Phytochemical Analysis of Methanolic Extract of Roots of Kalanchoe pinnata by HPLC and GCMS. Asian Journal of Research in Chemistry. 2011 ;4(4) :655-8.
33. Kumar G, Divya R, Pratyusha V, Monica G. Phytochemical Investigations on Bark and Invitro Microbial Assay of Zanthoxylum armatum DC. Asian Journal of Research in Chemistry. 2012 ;5(6):730-2.
34. Nikhat F, Satyanarayana D, Shastri C, Rajni S, Sheikh A. The Phytochemicals explored from the roots of Syzygium cuminni (L) skeel assessed for Anti-hyperglycemic activity. Asian Journal of Research in Chemistry. 2013 ;6(10):920-5.
35. Jain S, Kar A. The antibacterial activity of some essential oils and their combinations. Planta Medica. 1971 ;20(04):118-23.
36. Shaikh T, Mujum A, Wasimuzzama K, Rub RA. Pharmacognostic and Preliminary Phytochemical Screening of Cichorium intybus Linn Seed. Asian Journal of Research in Chemistry. 2010 ;3(4): 977-80.