0974-4150 (Online)
0974-4169 (Print)

Author(s): Abdelhakim Benarima, Salah Eddine Laouini, Mohamed Nasreddine Raache, Moulay Rachid Kouadri


DOI: 10.5958/0974-4150.2021.00022.5   

Address: Abdelhakim Benarima1*, Salah Eddine Laouini1, Mohamed Nasreddine Raache2, Moulay Rachid Kouadri2
1Department of Process Engineering and Petrochemistry, Faculty of Technology, University of Echahid Hamma Lakhdar, El-Oued, BP 789, El-Oued, 39000, Algeria.
2Process Engineering Laboratory (PEL), Kasdi Merbah University, Ouargla, 30000, Algeria.
*Corresponding Author

Published In:   Volume - 14,      Issue - 2,     Year - 2021

Moringa oleifera leaves has gained much importance in recent days due to its multiple uses and benefits to the pharmaceutical and food industries. In the present study, the effect of different temperatures (15, 30 and 45°C) on the extraction of phenolic compounds and antioxidant capacity from Moringa oleifera leaves was studied used ultrasonic-assisted extraction as a modern method. The experimental results showed that 30(°C) achieved the maximum rate of extraction yield, phenolic content, flavonoid content, flavanols content, FRAP, antioxidant total and IC50 with values of 24.25%, 23.68 ±1.1 (mg GAE/100mg DW), 58.65 ±2.4 (mg RE/g DW), 10.04 ±0.21 (mg QE/g DW), 236.37 ±0.5 (mg FeSO4 E/mg DW), 72.26 ±1.2 (mg GAE/g DW) and 45.04 ±1.1 (µg/ml) respectively. Based on these results, we can conclude that temperature plays an important role to determine the amount of phenolic compounds extracted and antioxidant capacity during the extraction process.

Cite this article:
Abdelhakim Benarima, Salah Eddine Laouini, Mohamed Nasreddine Raache, Moulay Rachid Kouadri. Influence of Extraction temperature on the Phenolic compounds and Antioxidant Capacity from Moringa oleifera Leaves. Asian J. Research Chem. 2021; 14(2):120-124. doi: 10.5958/0974-4150.2021.00022.5

Abdelhakim Benarima, Salah Eddine Laouini, Mohamed Nasreddine Raache, Moulay Rachid Kouadri. Influence of Extraction temperature on the Phenolic compounds and Antioxidant Capacity from Moringa oleifera Leaves. Asian J. Research Chem. 2021; 14(2):120-124. doi: 10.5958/0974-4150.2021.00022.5   Available on:

1.    Mokrani A, Madani K. Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Sep Purif Technol. 2016; 162: 68-76.
2.    Reboredo-Rodríguez P, Varela-López A, Forbes-Hernández TY, et al. Phenolic compounds isolated from olive oil as nutraceutical tools for the prevention and management of cancer and cardiovascular diseases. Int J Mol Sci. 2018;19(8): 2305.
3.    Reddy MS, Kuber BR. Evaluation of Anti-Bacterial Activity of Leaf Extracts of Mimusops elengi and Moringa oleifera. Res J Pharmacogn Phytochem. 2016;8(1): 13-15.
4.    George M, Joseph L. Wound Healing and Anti-Bacterial Effects of Cassia auriculata Extract. Res J Pharmacol Pharmacodyn. 2010; 2(1): 48-51.
5.    Rastogi T, Ghorpade DS, Deokate UA, Khadabadi SS. Studies on antimicrobial activity of Boswellia serrata, Moringa oleifera and Vitex negundo: a comparison. Res J Pharmacogn Phytochem. 2009; 1(1): 75-77.
6.    Chand T, Sharma MK, Pandey FK, Chakraborty C. Anti-microbial activity of Ethanol extracts obtained from different parts of Calotropis gigantea. Res J Sci Technol. 2016; 8(3): 129-134.
7.    Rastogi T, Bhutda V, Moon K, Aswar PB, Khadabadi SS. Comparative studies on anthelmintic activity of Moringa oleifera and Vitex negundo. Asian J Res Chem. 2009; 2(2): 181-182.
8.    Dhimmar N, Patel NM, Gajera V, Lambole V. Pharmacological activities of Moringa oleifera: an overview. Res J Pharm Technol. 2015; 8(4): 476-480.
9.    Elayarani M, Shanmuganathan P, Muthukumaran P. In vitro anti-oxidant activity of the various extracts of Cassia auriculata L. flower by UV spectrophotometer. Asian J Pharm Technol. 2011; 1(3): 70-72.
10.    Shamim QM, Jitendra P, Reddy AV, Safiullah S, Mohapatra P. Phytochemicals and Pharmacological Activities of Moringa oleifera Lam. Res J Pharmacol Pharmacodyn. 2010; 2(2): 183-186.
11.    Narapusetty N, Sivaiah O, Balanasaraiah B, et al. Anti-Inflammatory activity of Ethanolic extract of Basella alba in acute and Sub-acute Model. Asian J Pharm Res. 2017; 7(2): 88-93.
12.    Manjula B, Hunasagi R, Shivalinge GKP. Anti-Obesity Activity of Ethanolic Extract of Moringa oleifera Seeds in Experimental Animals. Res J Pharmacol Pharmacodyn. 2011; 3(6): 318-328.
13.    Pavani M, Ramadurg B, Varshitha C. Anti-obesity Activities of Hydro-Alcoholic Extract of Echinochloa crusgalli (L.) P. Beauv Grains in Albino Rats. Res J Pharmacol Pharmacodyn. 2014; 6(1): 15-20.
14.    Karishma S, Lakshmi K, Tony DE, Babu AN, Nadendla RR. Pharmacological Evaluation of Leaf Extract of Terminalia bellerica with Moringa oleifera for its Synergistic Action on Anti-diabetic Activity and Anti-inflammatory Activity in Rats. Res J Pharm Technol. 2019;12(3): 1181-1184.
15.    Argade PA, Bhutkar MA, Magdum CS. Albizzia lebbeck extract mediated synthesis of Zinc Oxide Nanoparticles and study of its In-vitro Anti-diabetic and Anti-oxidant activity. Asian J Pharm Technol. 2019;9 (2): 93-98.
16.    Kähkönen MP, Hopia AI, Vuorela HJ, et al. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem. 1999; 47(10): 3954-3962.
17.    Panya T, Chansri N, Daodee S. Development and Evaluation of Lozenge from Moringa oleifera leaf extract. Res J Pharm Technol. 2016; 9(7): 805-809.
18.    Series IOPC, Science M. Enhanced Extraction of Phenolic Compounds from Moringa oleifera Leaves Using Subcritical Water Ethanol Mixture Enhanced Extraction of Phenolic Compounds from Moringa oleifera Leaves Using Subcritical Water Ethanol Mixture. IOP Conf Ser Mater Sci Eng. 2019; 543(1): 12021.
19.    Sundararaman S, Narendrakumar G, Sundari N, Amarnath M, Thayyil PJ. Extraction of Pectin from used Citrus Limon and optimization of process parameters using Response Surface Methodology. Res J Pharm Technol. 2016;9(12): 2246-2251.
20.    Amina B-B, Roukia H, Mahfoud HA, Ahlem T, Chahrazed B, Houria M. Optimization of Extraction conditions of the Polyphenols, Flavonoids and the Antioxidant activity of the plant Ammosperma cinereum (Brassicaceae) through the Response Surface Methodology (RSM). Asian J Res Chem. 2020; 13(1):1-6.
21.    Raul SK, Padhy GK, Charan SA, Gurubarik N. Comparative Evaluation of Anthelmintic Potential of Cucurbita maxima Seeds, Moringa oleifera Seeds and Solanum surattense Fruits. Res J Pharmacogn Phytochem. 2014; 6(4):187-189.
22.    Chen Y, Xie M-Y, Gong X-F. Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. J Food Eng. 2007;81(1): 162-170.
23.    Alara OR, Abdurahman NH, Ukaegbu CI. Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity. J Appl Res Med Aromat Plants. 2018; 11:12-17.
24.    Aktumsek A, Zengin G, Ozmen G, Selim Y. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. FOOD Chem Toxicol. 2013; 55: 290-296.
25.    Guemari F, Laouini SE, Rebiai A, Bouafia A. Phytochemical screening and Identification of Polyphenols, Evaluation of Antioxidant activity and study of Biological properties of extract Silybum marianum (L.). Asian J Res Chem. 2020; 13(3):190-197.
26.    Shahwar D, Raza MA. Antioxidant potential of phenolic extracts of Mimusops elengi. Asian Pac J Trop Biomed. 2012; 2(7): 547-550.
27.    Blandine L, Kenfack M, Bruntha P. Phenolic profile, antioxidant and enzyme inhibitory activities of underutilized spices from Central Africa. J Food Sci Technol. 2020:1-14.
28.    Oil LS, Panchal B, Deshmukh S, Sharma M. Optimization of Oil Extraction and Characterization from Tamarindus Indica Optimization of oil extraction and characterization from tamarindus indica Linn seed oil. Int J Oil, Gas Coal Eng. 2014; 2(1):1.
29.    Braga MEM, Moreschi SRM, Meireles MAA. Effects of supercritical fluid extraction on Curcuma longa L. and Zingiber officinale R. starches. Carbohydr Polym. 2006; 63(3): 340-346.
30.    Doke S, Guha M. Identification of extraction conditions for determination of phenolic contents of garden cress seed (Lepidium sativum L.) and its milled fractions. Food Anal Methods. 2015;8(4): 1053-1057.
31.    Hui CK, Zainol K, Zin ZM. Preliminary phytochemical screening and effect of hot water extraction conditions on phenolic contents and antioxidant capacities of Morinda citrifolia leaf. Malaysian Appl Biol. 2018; 47(4): 13–24.
32.    Réblová Z. Effect of temperature on the antioxidant activity of phenolic acids. Czech J Food Sci. 2012; 30(2): 171-175.
33.    Zhu K, Lian C, Guo X, Peng W, Zhou H. Antioxidant activities and total phenolic contents of various extracts from defatted wheat germ. Food Chem. 2011; 126(3): 1122-1126.

Recomonded Articles:

Author(s): Syed Tasleem Hussain, Gul Abad Khan, Muhammad Shabeer

DOI:         Access: Open Access Read More

Author(s): Kateryna Taran, Ayaou Abderrahim, Vera Kravchenko, Olena Novosel, Svitlana Taran

DOI: 10.5958/0974-4150.2020.00034.6         Access: Open Access Read More

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Alka Verma, Bhupesh Verma, Sunil Kumar Prajapati, Kishu Tripathi

DOI:         Access: Open Access Read More

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): PA Jadhav, CS Raut, JP Bidada, BB Buwa, PN Dhabale, SC Dhawale

DOI:         Access: Open Access Read More

Author(s): Banerjee S, Bonde CG, Merukar SS, Patil YR

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles

Recent Articles