ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Bernard W. Makau, Paul K. M. Sang, Charles O. Oloo

Email(s): bernardwambua0463@gmail.com

DOI: 10.52711/0974-4150.2023.00070   

Address: Bernard W. Makau, Paul K. M. Sang, Charles O. Oloo
Department of Chemistry, School of Science, Dedan Kimathi University of Technology, Kenya.
*Corresponding Author

Published In:   Volume - 16,      Issue - 6,     Year - 2023


ABSTRACT:
Milk adulteration is done to increase the milk quantity or its shelf life. This research focused on the latter, accomplished by adding chemicals such as formalin. This is a great global concern and it causes detrimental effects such as cancer, myeloid leukemia. The chemical effects of formalin on milk include reducing its pH, protein, and fat percentage. Five samples were collected from several milk vendors around Nyeri Municipality and a sample directly from the cow. The pure milk had the fastest reduction time (18.3±1.53minutes) in Methylene Blue Reduction Test. The milk samples had an average of 3.3929±2.0579ppm formalin. In UV-Vis, the LOD and LOQ, R2 were 0.001M and 0.004M, 0.9868 respectively. Both the butter fat, and pH values decreased with formalin concentration increase. Their mean was 3.1±0.4% and 5.47 ± 0.806 respectively. Out of the six samples, 83.33% of them were adultered with formalin.


Cite this article:
Bernard W. Makau, Paul K. M. Sang, Charles O. Oloo. Determination of the Presence, Concentration, and effects of Formalin on Raw milk around Nyeri Municipality. Asian Journal of Research in Chemistry. 2023; 16(6):429-2. doi: 10.52711/0974-4150.2023.00070

Cite(Electronic):
Bernard W. Makau, Paul K. M. Sang, Charles O. Oloo. Determination of the Presence, Concentration, and effects of Formalin on Raw milk around Nyeri Municipality. Asian Journal of Research in Chemistry. 2023; 16(6):429-2. doi: 10.52711/0974-4150.2023.00070   Available on: https://ajrconline.org/AbstractView.aspx?PID=2023-16-6-6


REFERENCES:
1.     Upadhyay N, Goyal A, Kumar A, Ghai DL, Singh R. Preservation of milk and milk products for analytical purposes. Food Rev Int. 2014; 30(3): 203-224.
2.     Furia TE. CRC Handbook of Food Additives. Vol 1. CRC Press; 1973.
3.     Catsberg CME, Dommelen GJM. Methods of preservation. In: Food Handbook. Springer; 1990:42-57.
4.     Soffritti M, Belpoggi F, Lambertin L, Lauriola M, Padovani M, Maltoni C. Results of long‐term experimental studies on the carcinogenicity of formaldehyde and acetaldehyde in rats. Ann N Y Acad Sci. 2002; 982(1): 87-105.
5.     Zhang L, Steinmaus C, Eastmond DA, Xin XK, Smith MT. Formaldehyde exposure and leukemia: a new meta-analysis and potential mechanisms. Mutat Res Mutat Res. 2009; 681(2-3): 150-168.
6.     Singh P, Gandhi N. Milk preservatives and adulterants: processing, regulatory and safety issues. Food Rev Int. 2015; 31(3):236-261.
7.     Jenkins TC, McGuire MA. Major advances in nutrition: impact on milk composition. J Dairy Sci. 2006; 89(4): 1302-1310.
8.     McLaughlin JK. Formaldehyde and cancer: a critical review. Int Arch Occup Environ Health. 1994; 66(5): 295-301.
9.     Blair A, Stewart P, O’Berg M, et al. Mortality among industrial workers exposed to formaldehyde. J Natl Cancer Inst. 1986; 76(6): 1071-1084.
10.     Handford CE, Campbell K, Elliott CT. Impacts of milk fraud on food safety and nutrition with special emphasis on developing countries. Compr Rev Food Sci Food Saf. 2016; 15(1): 130-142.
11.     McClements DJ, Newman E, McClements IF. Plant‐based milks: A review of the science underpinning their design, fabrication, and performance. Compr Rev Food Sci Food Saf. 2019; 18(6): 2047-2067.
12.     Ranvir S, Gosewade S, Kumar H, Seth R. Effect of methyl paraben, propyl paraben and formalin preserved milk on chemical composition during storage. Orient J Chem. 2015; 31(4): 2147-2152.
13.     Bapat P, Kumar Nandy S, Wangikar P, Venkatesh K V. Place of the Methylene Blue and Resazurin Reduction Tests in a Milk Control Program. Am J Public Heal Nations Heal. 1939; 29(3): 239-247. doi:10.2105/AJPH.29.3.239
14.     Nag S, Das D, Tudu B, Roy RB. Multivariate Analysis of Formalin Using UV-Vis Spectroscopy. In: 2021 IEEE Second International Conference on Control, Measurement and Instrumentation (CMI). IEEE. 2021:133-137.
15.     Chanda T, Debnath GK, Hossain ME, Islam MA, Begum MK. Adulteration of raw milk in the rural areas of Barisal district of Bangladesh. Bangladesh J Anim Sci. 2012; 41(2):112-115.
16.     Thornton H, Strynadka N,  1934  undefined. Milk contamination and the methylene blue reduction test. JSTOR. Accessed June 9, 2022. https://www.jstor.org/stable/41979309
17.     Thornton HR, Sandin RB. Standardization of the Methylene Blue Reduction Test by the Use of Methylene Blue Thiocyanate. Am J Public Heal Nations Heal. 1935; 25(10): 1114-1117. doi:10.2105/AJPH.25.10.1114
18.     Förster H. UV/Vis spectroscopy. In: Characterization I. Springer; 2004:337-426.
19.     Mäntele W, Deniz E. UV–VIS absorption spectroscopy: Lambert-Beer reloaded. Spectrochim Acta Part A Mol Biomol Spectrosc. 2017; 173: 965-968.
20.     Yuan C, Pu J, Fu D, Min Y, Wang L, Liu J. UV-vis Spectroscopic Detection of Formaldehyde and its Analogs: A Convenient And Sensitive Methodology. J Hazard Mater. Published online 2022: 129457.
21.     Ikonen T, Morri S, Tyrisevä AM, Ruottinen O, Ojala M. Genetic and phenotypic correlations between milk coagulation properties, milk production traits, somatic cell count, casein content, and pH of milk. J Dairy Sci. 2004; 87(2): 458-467.
22.     Tomovska J, Gjorgievski N, Makarijoski B. Examination of pH, Titratable Acidity and Antioxidant Activity in Fermented Milk, Journal of materials Science and Engineering. J Mater Sci Eng A. 2016; 6(11): 326-333.
23.     Bailey DE. Study of Babcock test for butterfat in milk. J Dairy Sci. 1919;2(5):331-373.
24.     Kleyn DH, Lynch JM, Barbano DM, Bloom MJ, Mitchell MW. Determination of fat in raw and processed milks by the Gerber method: collaborative study. J AOAC Int. 2001; 84(5):1499-1508.
25.     Radema L, Mulder H. Methods for determining the fat content of milk. 2. The Gerber method. Ned Melk-en Zuiveltijdschr. 1948; 2: 210-222.
26.     Kleyn DH, Trout JR, Weber M. Determination of fat in raw milk: comparison of mojonnier (ether extraction) and Gerber method. J Assoc Off Anal Chem. 1988; 71(4): 851-853.
27.     Picollo M, Aceto M, Vitorino T. UV-Vis spectroscopy. Phys Sci Rev. 2019; 4(4).
28.     Journal HTCPH, 1933  undefined. Laboratory Section: The Use of the Methylene Blue Reduction Test. JSTOR. Accessed June 9, 2022. https://www.jstor.org/stable/41976838
29.     Rohyami Y, Pribadi RM. Validation of methods on formalin testing in tofu and determination of 3, 5-diacetyl-dihydrolutidine stability by UV-Vis spectrophotometry. In: AIP Conference Proceedings. Vol 1911. AIP Publishing LLC; 2017: 20018.
30.     Wahba NM, Korashy EA. A preliminary detection of inhibitory substances in milk sold in Assiut Governorate. Assiut Vet Med J. 2006; 52(109): 93-100.
31.     Thresh JC, Porter AE. Preservatives in Food and Food Examination. J. & A. Churchill; 1906.
32.     Rysstad G, Kolstad J. Extended shelf life milk—advances in technology. Int J dairy Technol. 2006; 59(2): 85-96.
33.     Upadhyay PK, Dayal R, Dwivedi HB. Physical and chemical quality of milk sold in Kanpur city (UP). Progress Agric. 2003; 3(1and2): 40-42.
34.     Choudhary A, Gupta N, Hameed F, Choton S. An overview of food adulteration: Concept, sources, impact, challenges and detection. Int J Chem Stud. 2020; 8(1): 2564-2573.














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