ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Shinde G. S., Jadhav R. S., Kote R. B., Kadam A. J., Bankar A. A.

Email(s): shinde.ganesh904@gmail.com

DOI: 10.52711/0974-4150.2024.00019   

Address: Shinde G. S.1*, Jadhav R. S. 1, Kote R. B.1, Kadam A. J.2, Bankar A. A.2
1Department of Pharmaceutics, Institute of Pharmacy Loni. Tal-Rahata, District - Ahmednagar. (M.S.) 413736.
2Department of Pharmaceutical Chemistry, Institute of Pharmacy Loni, Taluka-Rahata District-Ahmednagar. (M.S.) 413736.
*Corresponding Author

Published In:   Volume - 17,      Issue - 2,     Year - 2024


ABSTRACT:
Purpose: To develop a simple, sensitive, accurate, precise and rapid UV spectrophotometric method for the determination of metformin in human urine. Methods: Extraction of metformin from urine samples was done by Dilute and shoot method in which urine was diluted up to 1:10 ratio to avoid interference of matrix. For estimation of Metformin hydrochloride, the solvent system employed was water. The metformin hydrochloride was scanned in range of 200nm to 400 nm and wavelength of detection (?det) was found to be 234 nm. Result: The calibration curve was linear in the range of 1-17 µg/ml. the recovery and assay studies of metformin hydrochloride were within 93-94.85% indicating that the proposed method can be estimation of metformin hydrochloride


Cite this article:
Shinde G. S., Jadhav R. S., Kote R. B., Kadam A. J., Bankar A. A.. Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample. Asian Journal of Research in Chemistry. 2024; 17(2):93-6. doi: 10.52711/0974-4150.2024.00019

Cite(Electronic):
Shinde G. S., Jadhav R. S., Kote R. B., Kadam A. J., Bankar A. A.. Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample. Asian Journal of Research in Chemistry. 2024; 17(2):93-6. doi: 10.52711/0974-4150.2024.00019   Available on: https://ajrconline.org/AbstractView.aspx?PID=2024-17-2-7


REFERENCE:
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2.    Grzybowska M, Bober J, Olszewska M. Metformin - mechanisms of action and use for the treatment of type 2 diabetes mellitus. Postepy Hig Med Dosw online. 2011; 6(65): 277-85.
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