Babu Ganesan, Perumal Perumal, Vijaya Baskaran Manickam, Surya Rao Srikakolapu, Srujana Divya Gotteti, Latha Sundaresan Thirumurthy
Babu Ganesan1*, Perumal Perumal1, Vijaya Baskaran Manickam1, Surya Rao Srikakolapu1, Srujana Divya Gotteti1 and Latha Sundaresan Thirumurthy2
1Department of Pharmaceutical Analysis and Chemistry, J. K. K. Nataraja College of Pharmacy, Komarapalayam 638183 Namakkal District, TamilNadu
2Department of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Tiruchengode 637205 Namakkal District, TamilNadu
Volume - 3,
Issue - 3,
Year - 2010
A simple, precise and accurate reverse phase HPLC method has been established for simultaneous quantitative determination of strychnine and brucine in herbal formulation (tablets). The HPLC method was performed on a Phenomenex C-18 ODS column (250 mm × 4.6 mm ID, 5 µm), with isocratic elution using a mixture of acetonitrile: phosphate buffer in the ratio of 15: 85 v/v at flow rate of 1 mL/min with UV detection at 264 nm. Strychnine and brucine were well resolved on the stationary phase, and the retention times were 6.263 minutes for strychnine and 4.987 minutes for brucine. The calibration curves were found to be linear (Correlation coefficient, r = 0.9997 for strychnine and 0.9996 for brucine) in the concentration range 20-120 µg/mL for strychnine and brucine. The herbal preparation was quantified by external standard calibration method. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination of strychnine and brucine in herbal formulation.
Cite this article:
Babu Ganesan, Perumal Perumal, Vijaya Baskaran Manickam, Surya Rao Srikakolapu, Srujana Divya Gotteti, Latha Sundaresan Thirumurthy. Simultaneous Determination of Strychnine and Brucine in Herbal Formulation by RP-HPLC. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 720-723.