ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Vishal Jain, Ambar Vyas, Swarnlata Saraf, S. Saraf

Email(s): vj_rsofiop@rediffmail.com

DOI: Not Available

Address: Vishal Jain*, Ambar Vyas, Swarnlata Saraf and S. Saraf
Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (CG)
*Corresponding Author

Published In:   Volume - 4,      Issue - 2,     Year - 2011


ABSTRACT:
Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Ayurvedic medicines/herbal formulations. ‘Trikatu churna’ is one of the oldest and popular Ayurvedic preparations, is official in Ayurvedic formulary of India used widely for disorder of respiratory tract and digestive system. It comprised of the fruits Piper longum (Pippali), Piper nigrum (Marica) and rhizomes of Zingiber officianalis (Saunth). The present study is an attempt to develop the fingerprint method for Trikatu Churna by simple high-performance liquid chromatography (HPLC) determination using Piperine as a standard, which is as an important and major content in formulation. RP- HPLC methods for determination of Piperine from the fruits of Pippali, Marica and Trikatu Churna have been developed. A C 18 LUNA (5 micron 25 cm×4.6 mm) column from Phenomenex in binary gradient mode with mobile phase methanol at flow rate is 1.0ml/min, and effluent was monitored at 342 nm. Validation of the method was done with a view to demonstrate its selectivity, linearity, precision and accuracy. The concentration of Piperine present in raw material was found to be 4.1%± 0.42w/w in marica, and 2.05% ± 0.39w/w in pippali respectively and in three identical laboratory batch of Trikatu Churna name TK-I, TK-II, TK-III, was 2.02%±0.61, 2.23%±0.49, 2.21%±0.53w/w respectively with mean value 2.15%±0.54w/w. The Piperine content of all the three batches is found to be in close proximities with each other. Obtained results were compared with marketed formulations.


Cite this article:
Vishal Jain, Ambar Vyas, Swarnlata Saraf, S. Saraf. HPLC Determination of Piperine in ‘Trikatu Churna’ a Potent Ayurvedic Formulation for Routine Quality Control. Asian J. Research Chem. 4(2): February 2011; Page 183-186.


Recomonded Articles:

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Tauseef Shaikh, Atar Mujum , Khan Wasimuzzama, Rukhsana A Rub

DOI:         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): B. Balaswami, P. Venkata Ramana, B. Subba Rao, P. Sanjeeva

DOI: 10.5958/0974-4150.2018.00023.8         Access: Open Access Read More

Author(s): Ismail, R Rajavel, M Ganesh, M Jagadeeswaran, K Srinivasan, J Valarmathi, T Sivakumar

DOI:         Access: Open Access Read More

Author(s): JR Rao, Mohan Kumar, SS Yadav

DOI:         Access: Open Access Read More

Author(s): Effat Souri, Nazanin Shabani Ravari, Farhad Alvandifar, Arsalan Negahban Aghdami, Maliheh Barazandeh Tehrani, Massoud Amanlou

DOI:         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Muralidhar Reddy Avuthu, B. Venkata Reddy, SNVS Murthy

DOI: 10.5958/0974-4150.2016.00076.6         Access: Open Access Read More

Author(s): VB Kshirsagar, UA Deokate, VB Bharkad, SS Khadabadi

DOI:         Access: Open Access Read More

Author(s): Sravani A

DOI: 10.5958/0974-4150.2018.00144.X         Access: Open Access Read More

Author(s): K. Kishore Kumar, R.S.K. Sharma, P. Chanti Babu, M. Sreenivasa Rao, K. Durga Prasadu, D. Ravi Kumar

DOI: 10.5958/0974-4150.2017.00067.0         Access: Open Access Read More

Author(s): Prasanna Reddy Battu, MS Reddy

DOI:         Access: Open Access Read More

Author(s): Sachin R Shinde , Suvarna I Bhoir, Namdev S Pawar, Suman B Yadav, Ajay S Ghumatkar, Ashok M Bhagwat

DOI:         Access: Open Access Read More

Author(s): J Vanitha, A Saravana Kumar, M Ganesh, VS Saravanan

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags