ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Mahmoud M. Sebaiy, Abdullah A. El-Shanawany, Sobhy M. El-Adl, Lobna M. Abdel-Aziz, Hisham A. Hashem

Email(s): sebaiy_pharma@yahoo.com

DOI: Not Available

Address: Mahmoud M. Sebaiy1, Abdullah A. El-Shanawany1, Sobhy M. El-Adl1, Lobna M. Abdel-Aziz1 and Hisham A. Hashem2.
1Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Egypt.
2Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Egypt.
*Corresponding Author

Published In:   Volume - 4,      Issue - 11,     Year - 2011


ABSTRACT:
An isocratic RP-HPLC method had been developed for rapid simultaneous separation and determination of four fluoroquinolones including levofloxacin HCl, lomefloxacin HCl, gatifloxacin and sparfloxacin in pure form or in presence of some impurities within 5 minutes. Separation was carried out on a Chromolith® Performance RP-18e (100 x 4.6 mm) using a mobile phase of MeOH : 0.025M KH2PO4 adjusted to pH 3 using ortho - phosphoric acid (20:80, v/v) at ambient temperature. The flow rate was 4 ml/min and maximum absorption was measured at 290 nm. The standard curve was linear in the concentration range of 1-80 µg/mL for all drugs. The retention time of levofloxacin HCl, lomefloxacin HCl, gatifloxacin and sparfloxacin was noted to be 1.11, 2, 2.98, and 4.3 minutes respectively, indicating shorter analysis time. The method was validated according to ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent. It was successfully applied for the analysis of these drugs in marketed formulations and could be effectively used for the routine analysis of formulations containing any one of the above drugs, or a combination, without any alteration in the chromatographic conditions.


Cite this article:
Mahmoud M. Sebaiy, Abdullah A. El-Shanawany, Sobhy M. El-Adl, Lobna M. Abdel-Aziz, Hisham A. Hashem. Rapid RP-HPLC Method for Simultaneous Estimation of Levofloxacin Hydrochloride, Lomefloxacin Hydrochloride, Gatifloxacin and Sparfloxacin. Asian J. Research Chem. 4(11): Nov., 2011; Page 1688-1694.


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