Jiby Elias, Rajesh M.G., Anish N.P., Manu M.S., Iwin C. Varkey
Jiby Elias1, Rajesh M.G.1*, Anish N.P.1, Manu M.S.2 and Iwin C. Varkey2
1Department of Biotechnology, Navajyothi Sree Karunakara Guru Research Centre for Ayurveda and Siddha, Uzhavoor, Kottayam, Kerala, India – 686 634
2CMS College of Science and Commerce, Coimbatore, Tamil Nadu, India – 641006
Volume - 4,
Issue - 3,
Year - 2011
In vitro antioxidant activities and preliminary phytochemical profiling of the methanol extract of the stem bark of Terminalia chebula Retz. (METC) of Combretaceae family were investigated. Total reducing power of the extract increased with increasing concentration which indicated that the plant has inherent antioxidant potential. The extract at a concentration of 500µg/ml showed maximum activity (85.72%) for 2, 2-diphenyl- 2- picryl-hydrazyl (DPPH) followed by nitric oxide (82.28%), superoxide (79.12%) and hydroxyl (77.28%) radicals. The IC50 values of DPPH, nitric oxide, superoxide and hydroxyl radicals were 72µg/ml, 73µg/ml, 69µg/ml and 80µg/ml respectively. METC had 28.6mg phenols per gram. METC showed the presence of phytoconstituents such as alkaloids, terpenoids, saponins, quinines, anthroquinones, tannins, carbohydrates, volatile oils, and phenolics. However, glycoside, coumarins, reducing sugars and resins were absent. Thin layer chromatogram showed two spots (Rf: 0.41, 0.56) under visible light and five spots (Rf: 0.41, 0.49, 0.56, 0.62, 0.71) on exposure to Iodine vapour. The findings suggest that the methanol extract of T. chebula possesses significant free radical scavenging activities in different in vitro models and could be a potent source of natural antioxidants.
Cite this article:
Jiby Elias, Rajesh M.G., Anish N.P., Manu M.S., Iwin C. Varkey. Terminalia chebula Retz. Stem Bark Extract: A Potent Natural Antioxidant. Asian J. Research Chem. 4(3): March 2011; Page 445-449.