Asian Journal of Research in Chemistry

ISSN

0974-4150 (Online)
0974-4169 (Print)


Submit Article

Confirmatory Method for Determination of 11-Nor-Δ9 -Tetrahydrocannabinol-9-Carboxylic Acid in Urine Samples Using Gas Chromatography–Mass Spectrometry (GC/MS)

Author(s): Rabie S. Farag, Sayed R. Abed Al-Salam

Email(s): sayed_ramadan100@yahoo.com

DOI: Not Available

Address: Rabie S. Farag1*, Sayed R. Abed Al-Salam2
1Prof. of Inorganic Chemistry, Al-Azhar University, Cairo, Egypt
2Drugs and Toxicology Expert, Medico-Legal Organization, Cairo, Egypt
*Corresponding Author

Published In:   Volume - 4,      Issue - 3,     Year - 2011

 View HTML        View PDF

ABSTRACT:
Gas chromatography mass spectrometry (GC/MS) analysis of 11-Nor-?9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) and the major metabolite of tetrahydrocannabinol in biological samples is reported The THC metabolite obtained by alkaline hydrolysis from urine samples were extracted using mixture of solvents followed by trimethylsilylation. The derivatized extract was submitted to GC/MS analysis of EI-SIM mode. Different factors were studied as pH, temperature and time to establish the best conditions for the determination. The calibration curves of THC-COOH derivatized (THC-COOH-TMS) in urine samples were linear in the concentration range from 10 to 150 ng/ml. The proposed method is able to determine the major metabolite of cannabis derivatives at very low level (10 ng/ml) with high specificity so is analytical procedure can be used as confirmatory method in drug testing of cannabis use.


Keywords:

Cite this article:
Rabie S. Farag, Sayed R. Abed Al-Salam. Confirmatory Method for Determination of 11-Nor-Δ9 -Tetrahydrocannabinol-9-Carboxylic Acid in Urine Samples Using Gas Chromatography–Mass Spectrometry (GC/MS). Asian J. Research Chem. 4(3): March 2011; Page 373-376.

Cite(Electronic):
Rabie S. Farag, Sayed R. Abed Al-Salam. Confirmatory Method for Determination of 11-Nor-Δ9 -Tetrahydrocannabinol-9-Carboxylic Acid in Urine Samples Using Gas Chromatography–Mass Spectrometry (GC/MS). Asian J. Research Chem. 4(3): March 2011; Page 373-376.   Available on: https://ajrconline.org/AbstractView.aspx?PID=2011-4-3-7


Recomonded Articles:

Herbal tea for the treatment of Urinary diseases as Potent diuretic and Anti-inflammatory agent

Author(s): Kateryna Taran, Ayaou Abderrahim, Vera Kravchenko, Olena Novosel, Svitlana Taran

DOI: 10.5958/0974-4150.2020.00034.6         Access: Open Access Read More

Method Development and Validation for Simultaneous Estimation of Metamizole Sodium and Pitofenone HCl by a Stability Indicating RP-HPLC

Author(s): R. Vijayalakshmi, S. Anbazhagan

DOI:         Access: Open Access Read More

Forced degradation study of Terbinafine HCl by HPLC with PDA detector

Author(s): Tentu Nageswara Rao, Muralidhar Reddy Avuthu, B. Venkata Reddy, SNVS Murthy

DOI: 10.5958/0974-4150.2016.00076.6         Access: Open Access Read More

A Review Article on Flash Chromatography

Author(s): Sravani A

DOI: 10.5958/0974-4150.2018.00144.X         Access: Open Access Read More

A New Simple RP – HPLC Method for Simultaneous Estimation of Meropenem and Vaborbactam in Tablet Dosage Form

Author(s): B. Balaswami, P. Venkata Ramana, B. Subba Rao, P. Sanjeeva

DOI: 10.5958/0974-4150.2018.00023.8         Access: Open Access Read More

Stability Indicating RP- HPLC Method for Simultaneous Estimation of Valsartan and Amlodipine in Capsule Formulation.

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Detection of Commonly used Carbamates by Thin Layer Chromatography and Spot Test Method

Author(s): Subhasish Chaudhur, Biswajit Chandra Das

DOI:         Access: Open Access Read More

Pharmacognostic and Preliminary Phytochemical Screening of Cichorium intybus Linn Seed

Author(s): Tauseef Shaikh, Atar Mujum , Khan Wasimuzzama, Rukhsana A Rub

DOI:         Access: Open Access Read More

Critical review: Significance of Force degradation study with respect to current Pharmaceutical Scenario

Author(s): Nikita N. Patel, Charmy S. Kothari

DOI:         Access: Open Access Read More

Gas Chromatography Mass Spectrometry Analysis of Andrographis paniculata

Author(s): N. Prabha, J. Rahmath Bushra

DOI: 10.5958/0974-4150.2019.00001.4         Access: Open Access Read More

Synthesis, characterization and pharmacological evaluation of novel spiro heterocyclic compounds as anti diabetic agents

Author(s): K. Kishore Kumar, R.S.K. Sharma, P. Chanti Babu, M. Sreenivasa Rao, K. Durga Prasadu, D. Ravi Kumar

DOI: 10.5958/0974-4150.2017.00067.0         Access: Open Access Read More

Isolation and Structure Characterization of related impurity in Olanzapine key starting material by LC/ESI-MS and NMR

Author(s): Jagadeesh Narkedimilli, Vishweshwar Vavilala, Sandeep Mohanty, Saravanan Manvalan, Jayashree Anireddy

DOI: 10.5958/0974-4150.2018.00096.2         Access: Open Access Read More

Simultaneous Determination of Strychnine and Brucine in Herbal Formulation by RP-HPLC

Author(s): Babu Ganesan, Perumal Perumal, Vijaya Baskaran Manickam, Surya Rao Srikakolapu, Srujana Divya Gotteti, Latha Sundaresan Thirumurthy

DOI:         Access: Open Access Read More

Phyto-Physico chemical evaluation, Antioxidant activities and Diuretic activity of Leaves of Lagerstroemia reginae

Author(s): S Kalidas, B Kameswari, P Devi, B Madhumitha, R Meera, NJ Merlin

DOI:         Access: Open Access Read More

Simultaneous Determination of Sodium Benzoate and Potassium Sorbate Preservatives in Foodstuffs Using High-Performance Liquid Chromatography

Author(s): Antakli S, Alahmad A, Badingki H

DOI:         Access: Open Access Read More

Analytical Method Development and Validation of Cefixime and Dicloxacillin Tablet by RP-HPLC

Author(s): Kathiravan S., Anbarasi B., Mathankumar S.

DOI:         Access: Open Access Read More

Phytochemical screening and column chromatography studies of Aerva lanata

Author(s): A. Anita, A. Malar Retna, J. Joseph

DOI: 10.5958/0974-4150.2018.00019.6         Access: Open Access Read More

Analytical Methods Validation for Estimation of Squalene from the Rajgeera Seed (Amaranth paniculata)

Author(s): Omprakash H. Nautiyal, Krishna K. Tiwari, Rekha S. Singhal

DOI:         Access: Open Access Read More

Synthesis of Some Novel Benzotriazole-1-yl-acetic acid substituted aryl hydrazide Derivatives as Anticonvulsant Agents

Author(s): B. Pochaiah, C.P. Meher, B. Srujana, P. Swarnalatha, A.Muralidhar Rao

DOI:         Access: Open Access Read More

Chemical and Microscopic Standardization of Poly-Herbal Unani Drug Sufoof-E-Hazim

Author(s): Asma S.K, Rashid M.A, Qasim M.A, Shoeb A.A, Afzal H, Mustehasan, Garg G.P.

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Quick Links

Latest Issues

Popular Articles


Recent Articles



Activated carbon from macadamia nutshells for removal of p-Nitrophenol from real wastewater: Thermodynamic evaluation
Review on Colon Targeted Drug Delivery System
Exploring the Effects of Polyacrylic Acid on Triton-X-100 Aqueous Solutions: Density, Viscosity and Ultrasonic Velocity Analysis
Formulation and Evaluation of Amaranthus dubius herbal gel
Estimation of Imatinib Mesylate by Extractive Colorimetric Methods in Bulk and Pharmaceutical Dosage Form
Qualitative and Quantitative Analysis of the Phytochemicals in Aerva lanata
Co-Solvent effect and Kinetics of Alkaline Hydrolysis of Butyle Salicylate
A Systematic Review on Ethnobotanical and Pharmacological Aspects of Croton plant Codiaeum variegatum (L.)
Review on Thiazolo-Triazole as a Promising Anticancer agent
Inimical effects of Monosodium Glutamate on various systems
Synthesis and Surface properties of LABSA Based detergents: An Ecofriendly Approach
A mini Review on Analytical and Bioanlytical Techniques in Study of Drugs-Protein Interaction
Review on Vesicular Drug Delivery System: Aquasomes
Synthesis, Characterization and Antioxidant Activity of some New Antipyrine derived Schiff Bases
Determination of Imatinib mesylate from Pharmaceutical Dosage Form by extractive ion pair complex colorimetric Method
Coupling of Single-Stranded poly-T DNA with Small Heterocyclic compounds via Peptide Linkage
Determination of Methdilazine by Extractive Colorimetric Method from Pharmaceutical Dosage Form
Synthesis and Surface properties of AOS Based detergents: A Green Synthetic approach
Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample
Oxovanadium (IV) Complexes of α-Amino Acid Schiff Bases and 2,2

Tags


About Journal

Asian Journal of Research in Chemistry (AJRC) (ISSN: print-0974-4169, Online-0974-4150) is an international, peer-reviewed journal devoted to pure and applied chemistry. AJRC publishes Original Research Articles, Short Communications, Review Articles in all aspects of chemistry. Topics covered including the traditional areas of analytical, inorganic, organic, biochemistry, forensic, physical-theoretical chemistry as well as newer interdisciplinary areas such as agriculture, materials science, computational, medicine, spectroscopy, polymers, supramolecular, surface, chemical physics, biological, medicinal/ drugs, environmental and pharmaceutical chemistry.
The journal is published quarterly every year in last week of March, June, September and December. From January 2011, is monthly and shall be published every year in last week of Month.    
Read More  

Visitors

Today: 821
Yesterday: 1438
Total: 1599217