ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): K S Chandrashekharaiah, H P Shweta, Rajiv Bharadwaj, N G Raju, N. Ramachandra Swamy

Email(s): kschandraks@gmail.com

DOI: 10.5958/0974-4150.2018.00063.9   

Address: K S Chandrashekharaiah1*, H P Shweta1, Rajiv Bharadwaj, N G Raju1,2, N. Ramachandra Swamy3
1Department of Biochemistry, Mangalore University, Chikka Aluvara, Kodagu-571232
2Department of Biotechnology, Karnataka State Open University, Mysore-570006
3Department of Biochemistry, Bangalore University, Bangalore-560001
*Corresponding Author

Published In:   Volume - 11,      Issue - 2,     Year - 2018


ABSTRACT:
Manifestations of metabolic disorders are greatly influenced by proteases in either up or down regulation of related biomarkers. Inhibition of these potential proteases results in better management of systemic disorders. Medicinal plants like Senna alata has been a great source of such protease inhibitors in which the current study was focused to isolate, purify and characterize inhibitors, more specifically, trypsin inhibitors. The protease inhibitors were isolated and purified employing conventional protein purification such as salt precipitation, gel filtration chromatography on Sephadex G-10 and G-50 and RP-HPLC. Two Senna alata protease inhibitors (SAPI-I and SAPI-II) were purified. SAPI-I was purified to 36.11 fold with a recovery of 42.80% and showed a specific inhibitor activity of 12.16. SAPI-II was purified to 41.23 fold with a recovery of 51.26 and showed a specific inhibitor activity of 19.61. Both the purified inhibitors were found to be stable in the pH range 5 – 8 and temperature between 4 – 65oC.


Cite this article:
K S Chandrashekharaiah, H P Shweta, Rajiv Bharadwaj, N G Raju, N. Ramachandra Swamy. Characterization of Protease inhibitors from the seeds of Senna alata. Asian J. Research Chem. 2018; 11(2):351-354. doi: 10.5958/0974-4150.2018.00063.9

Cite(Electronic):
K S Chandrashekharaiah, H P Shweta, Rajiv Bharadwaj, N G Raju, N. Ramachandra Swamy. Characterization of Protease inhibitors from the seeds of Senna alata. Asian J. Research Chem. 2018; 11(2):351-354. doi: 10.5958/0974-4150.2018.00063.9   Available on: https://ajrconline.org/AbstractView.aspx?PID=2018-11-2-23


Recomonded Articles:

Author(s): Kateryna Taran, Ayaou Abderrahim, Vera Kravchenko, Olena Novosel, Svitlana Taran

DOI: 10.5958/0974-4150.2020.00034.6         Access: Open Access Read More

Author(s): Prathima Patil, S.P. Sethy, T. Sameena, K. Shailaja

DOI:         Access: Open Access Read More

Author(s): Tauseef Shaikh, Atar Mujum , Khan Wasimuzzama, Rukhsana A Rub

DOI:         Access: Open Access Read More

Author(s): Tripti Jain, V Jain, R Pandey, A Vyas, SS Shukla

DOI:         Access: Open Access Read More

Author(s): Alka Verma, Bhupesh Verma, Sunil Kumar Prajapati, Kishu Tripathi

DOI:         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Muralidhar Reddy Avuthu, B. Venkata Reddy, SNVS Murthy

DOI: 10.5958/0974-4150.2016.00076.6         Access: Open Access Read More

Author(s): Sravani A

DOI: 10.5958/0974-4150.2018.00144.X         Access: Open Access Read More

Author(s): Anshul Sinha, A. K. Meena, P. Panda, Bhavana Srivastava, M. D. Gupta, M. M. Padhi

DOI:         Access: Open Access Read More

Author(s): B. Balaswami, P. Venkata Ramana, B. Subba Rao, P. Sanjeeva

DOI: 10.5958/0974-4150.2018.00023.8         Access: Open Access Read More

Author(s): Hunasnalkar Shivraj G, Shaikh Gazi, Patil SM, Surwase Ulhas S

DOI:         Access: Open Access Read More

Author(s): K. Kishore Kumar, R.S.K. Sharma, P. Chanti Babu, M. Sreenivasa Rao, K. Durga Prasadu, D. Ravi Kumar

DOI: 10.5958/0974-4150.2017.00067.0         Access: Open Access Read More

Author(s): Manorama B. Motegaonkar, S. D. Salunke

DOI: 10.5958/0974-4150.2017.00025.6         Access: Open Access Read More

Author(s): Sheetal1, Sonika, Poonam Nandal, S.P. Khatkar, Ritu Langyan

DOI: 10.5958/0974-4150.2018.00001.9         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags